通过免疫组织化学对石蜡包埋的3D胶质瘤神经球培养物进行胶质瘤生物标志物评估。
Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures.
作者信息
Núñez Felipe J, Mendez Flor M, Garcia-Fabiani Maria B, Pardo Joaquín, Edwards Marta, Lowenstein Pedro R, Castro Maria G
机构信息
Department of Neurosurgery, University of Michigan Medical School; Department of Cell & Developmental Biology, University of Michigan.
Department of Cell & Developmental Biology, University of Michigan.
出版信息
J Vis Exp. 2019 Jan 9(143). doi: 10.3791/58931.
Analysis of protein expression in glioma is relevant for several aspects in the study of its pathology. Numerous proteins have been described as biomarkers with applications in diagnosis, prognosis, classification, state of tumor progression, and cell differentiation state. These analyses of biomarkers are also useful to characterize tumor neurospheres (NS) generated from glioma patients and glioma models. Tumor NS provide a valuable in vitro model to assess different features of the tumor from which they are derived and can more accurately mirror glioma biology. Here we describe a detailed method to analyze biomarkers in tumor NS using immunohistochemistry (IHC) on paraffin-embedded tumor NS.
胶质瘤中蛋白质表达的分析在其病理学研究的多个方面都具有相关性。许多蛋白质已被描述为生物标志物，可应用于诊断、预后、分类、肿瘤进展状态和细胞分化状态。这些生物标志物分析对于表征从胶质瘤患者和胶质瘤模型产生的肿瘤神经球（NS）也很有用。肿瘤神经球提供了一个有价值的体外模型，以评估其所源自肿瘤的不同特征，并且可以更准确地反映胶质瘤生物学特性。在这里，我们描述了一种详细的方法，用于在石蜡包埋的肿瘤神经球上使用免疫组织化学（IHC）分析肿瘤神经球中的生物标志物。
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