Paponov Ivan A, Dindas Julian, Król Elżbieta, Friz Tatyana, Budnyk Vadym, Teale William, Paponov Martina, Hedrich Rainer, Palme Klaus
Faculty of Biology, Institute of Biology II/Molecular Plant Physiology, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.
Norwegian Institute of Bioeconomy Research, Klepp, Norway.
Front Plant Sci. 2019 Jan 17;9:1953. doi: 10.3389/fpls.2018.01953. eCollection 2018.
Auxin is a molecule, which controls many aspects of plant development through both transcriptional and non-transcriptional signaling responses. AUXIN BINDING PROTEIN1 (ABP1) is a putative receptor for rapid non-transcriptional auxin-induced changes in plasma membrane depolarization and endocytosis rates. However, the mechanism of ABP1-mediated signaling is poorly understood. Here we show that membrane depolarization and endocytosis inhibition are ABP1-independent responses and that auxin-induced plasma membrane depolarization is instead dependent on the auxin influx carrier AUX1. AUX1 was itself not involved in the regulation of endocytosis. Auxin-dependent depolarization of the plasma membrane was also modulated by the auxin efflux carrier PIN2. These data establish a new connection between auxin transport and non-transcriptional auxin signaling.
生长素是一种通过转录和非转录信号反应来控制植物发育诸多方面的分子。生长素结合蛋白1（ABP1）被认为是介导生长素快速非转录诱导的质膜去极化和内吞作用速率变化的受体。然而，ABP1介导的信号传导机制仍知之甚少。在此，我们表明膜去极化和内吞作用抑制是不依赖ABP1的反应，相反，生长素诱导的质膜去极化依赖于生长素内流载体AUX1。AUX1本身不参与内吞作用的调节。质膜的生长素依赖性去极化也受到生长素外流载体PIN2的调节。这些数据建立了生长素运输与非转录生长素信号传导之间的新联系。
