Department of Civil and Environmental Engineering, University of Alberta, 7-263 Donadeo Innovation Centre for Engineering, Edmonton, Alberta T6G 1H9, Canada; College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
Department of Civil and Environmental Engineering, University of Alberta, 7-263 Donadeo Innovation Centre for Engineering, Edmonton, Alberta T6G 1H9, Canada.
Bioresour Technol. 2019 Apr;278:481-485. doi: 10.1016/j.biortech.2019.01.014. Epub 2019 Jan 6.
Source diverted blackwater (i.e., toilet wastewater with or without food waste residuals) represents an ideal waste stream for bioenergy recovery through anaerobic digestion. However, due to the high free ammonia concentration contained in blackwater collected from water conserving toilets (e.g., vacuum toilets), CH recovery can be significantly reduced because methanogenesis is inhibited. Our current study evaluated the feasibility of enriching hydrogenotrophic methanogens (known to be more resistant than acetoclastic methanogens toward free ammonia inhibition) in anaerobic sludge with H supplementation for blackwater treatment. Our results demonstrated that hydrogenotrophic methanogens can be enriched by supplementing anaerobic sludge with H and CO in a volume ratio of 4:1 for 3 months. The dominance of hydrogenotrophic methanogens after 3-month enrichment was confirmed with quantitative PCR studies. It was observed that the hydrogenotrophic methanogen dominant anaerobic sludge significantly alleviated blackwater free ammonia inhibition, and led to enhanced biochemical methane production (BMP) (e.g., from 30% to 53%). Our current study offers a new treatment option for anaerobic treatment of wastewater with high free ammonia content.
源污水（即含有或不含有食物残渣的厕所废水）通过厌氧消化回收生物能源是一种理想的废水。然而，由于从节水厕所（如真空厕所）收集的污水中含有高浓度的游离氨，沼气回收会显著减少，因为产甲烷作用受到抑制。本研究评估了在厌氧污泥中补充氢气（已知比乙酸营养型产甲烷菌对游离氨抑制更具抗性）以富集产氢产甲烷菌用于处理污水的可行性。研究结果表明，通过在 3 个月内以 H 和 CO 的体积比 4:1 补充氢气，可以富集产氢产甲烷菌。定量 PCR 研究证实了 3 个月富集后产氢产甲烷菌的优势地位。观察到产氢产甲烷菌优势厌氧污泥显著缓解了污水中的游离氨抑制作用，导致生化甲烷生成（BMP）增强（例如，从 30%增加到 53%）。本研究为高游离氨含量废水的厌氧处理提供了一种新的处理选择。
