在依诺沙星存在的情况下，紫外线A照射诱导HL-60细胞凋亡。
Apoptosis Induced by Ultraviolet A Exposure in the Presence of Enoxacin in HL-60 Cells.
作者信息
Shinada Haruka, Watanabe Takahiro, Okudaira Kazuho, Iwase Yumiko, Nishi Koji, Yumita Nagahiko
机构信息
Yokohama University of Pharmacy, Yokohama, Japan.
Yokohama University of Pharmacy, Yokohama, Japan
出版信息
Anticancer Res. 2019 Feb;39(2):687-693. doi: 10.21873/anticanres.13164.
BACKGROUND
The ultraviolet A (UVA) spectrum mainly includes the region associated with the phototoxicity of fluoroquinolone antimicrobial agents. This study investigated apoptosis induced with UVA light and enoxacin in HL-60 cells.
MATERIALS AND METHODS
HL-60 cells were irradiated by UVA (1.1 mW/cm) for 20 min in the presence or absence of enoxacin. The induction of apoptosis was investigated by analysing cell morphology, flow cytometry of annexin V-positive cells, DNA ladder formation, and caspase-3 activation.
RESULTS
Significant induction of apoptosis, DNA fragmentation, and caspase-3 activation were observed in cells treated with both UVA and enoxacin. UVA-induced apoptosis was significantly suppressed when NaN3, a singlet oxygen scavenger, was present.
CONCLUSION
Apoptosis was induced by the combination of UVA and enoxacin in HL-60 cells, and singlet oxygen appears to play an important role in photodynamically-induced apoptosis.
背景
紫外线A（UVA）光谱主要包括与氟喹诺酮类抗菌剂光毒性相关的区域。本研究调查了UVA光和依诺沙星诱导HL-60细胞凋亡的情况。
材料与方法
在存在或不存在依诺沙星的情况下，用UVA（1.1 mW/cm）照射HL-60细胞20分钟。通过分析细胞形态、膜联蛋白V阳性细胞的流式细胞术、DNA梯状条带形成和半胱天冬酶-3激活来研究凋亡的诱导情况。
结果
在用UVA和依诺沙星处理的细胞中观察到凋亡、DNA片段化和半胱天冬酶-3激活的显著诱导。当存在单线态氧清除剂NaN3时，UVA诱导的凋亡被显著抑制。
结论
UVA和依诺沙星联合诱导HL-60细胞凋亡，单线态氧似乎在光动力诱导的凋亡中起重要作用。
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