Du Xiangwei, Schrunk Dwayne E, Imerman Paula M, Smith Lori, Francis Kyle, Tahara John, Tkachenko Andriy, Reimschuessel Renate, Rumbeiha Wilson K
Iowa State University, Department of Veterinary Diagnostic and Production Animal Medicine, Veterinary Diagnostic Laboratory, 1850 Christensen Dr, Ames, IA 50011-1134.
MRIGlobal, 425 Volker Blvd, Kansas City, MO 64110.
J AOAC Int. 2019 Sep 1;102(5):1530-1534. doi: 10.5740/jaoacint.18-0355. Epub 2019 Feb 8.
Aflatoxins (AFs) are secondary metabolites of fungi and are one of the causes of toxin-related pet food recalls. An intralaboratory method was previously developed to quantify aflatoxin B (AFB) and aflatoxin M (AFM) in animal liver by HPLC with fluorescence detection. The aim of this study was to extensively evaluate the method performance with a single-laboratory blinded method test (BMT-S) and a multilaboratory blinded method test (BMT-M). Blinded tissue samples were prepared by a third-party laboratory and sent out to participating laboratories for both BMT-S and BMT-M. In both tests, participants analyzed blinded samples prepared by an independent laboratory. In the BMT-S, accuracy ranged between 111 and 154% for AFB and 113 and 159% for AFM within the quantitation range of 0.1-0.5 ng/g. The HorRat values for repeatability ranged between 0.1 and 0.3 for AFB and 0.3 and 0.6 for AFM. In the BMT-M, the interlaboratory accuracy ranged between 77 and 81% for AFB and 83 and 85% for AFM within the quantitation range of 0.2-10 ng/g. The HorRat values for reproducibility ranged between 0.4 and 0.7 for AFB and 0.4 and 0.9 for AFM. Both recovery and reproducibility were acceptable. BMT-M evaluation demonstrated that the method was suitable for quantitation of aflatoxins B and M in animal liver between laboratories. The BMT-S and BMT-M results demonstrated that the method is rugged and reproducible among the participating laboratories.
黄曲霉毒素（AFs）是真菌的次生代谢产物，也是导致与毒素相关的宠物食品召回的原因之一。此前已开发出一种实验室内部方法，通过高效液相色谱-荧光检测法对动物肝脏中的黄曲霉毒素B（AFB）和黄曲霉毒素M（AFM）进行定量分析。本研究的目的是通过单实验室盲法测试（BMT-S）和多实验室盲法测试（BMT-M）广泛评估该方法的性能。盲样组织由第三方实验室制备，并分发给参与BMT-S和BMT-M的实验室。在这两项测试中，参与者分析由独立实验室制备的盲样。在BMT-S中，在0.1 - 0.5 ng/g的定量范围内，AFB的准确度在111%至154%之间，AFM的准确度在113%至159%之间。AFB重复性的HorRat值在0.1至0.3之间，AFM的在0.3至0.6之间。在BMT-M中，在0.2 - 10 ng/g的定量范围内，AFB的实验室间准确度在77%至81%之间，AFM的在83%至85%之间。AFB再现性的HorRat值在0.4至0.7之间，AFM的在0.4至0.9之间。回收率和再现性均可接受。BMT-M评估表明该方法适用于不同实验室间对动物肝脏中黄曲霉毒素B和M的定量分析。BMT-S和BMT-M的结果表明该方法在参与的实验室中耐用且可重复。
