Time-Lapse Technologies and 4D Imaging of Kidney Development.
作者信息
Saarela Ulla, Skovorodkin Ilya
机构信息
Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland.
Laboratory of Developmental Biology, Biocenter Oulu, InfoTech, Oulu, Finland.
出版信息
Methods Mol Biol. 2019;1926:163-167. doi: 10.1007/978-1-4939-9021-4_14.
Time-lapse imaging is a technique of frequent imaging and following a course of a process. Because the development of the embryonic kidney can proceed ex vivo after dissection, it is possible to study the morphogenesis by culturing the kidney in the onstage incubator of a microscope and follow the developmental process by imaging. Confocal microscopes and other three-dimensional imaging systems offer the possibility for tracking the development process in four dimensions-3D and the time.
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