Qiao Q, Zhang Z-C, Qin Y-H, Zhang D-S, Tian Y-T, Wang Y-J
Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, Henan Province, 450002, China.
Plant Dis. 2011 Mar;95(3):356. doi: 10.1094/PDIS-09-10-0675.
Sweet potato chlorotic stunt virus (SPCSV) (genus Crinivirus, family Closteroviridae) is a whitefly-transmitted co-infectant of the sweet potato virus disease (SPVD), which can cause severe yield losses in sweet potato (Ipomoea batatas (L.) Lam.) (2,3). One isolate was obtained in Guangdong Province in August 2009 from sweet potato plants of cv. Guangshu No. 87 exhibiting symptoms typical of SPVD, including stunting, leaf distortion, vein clearing, and chlorosis. Leaf extracts from the symptomatic plants tested positive for SPCSV by nitrocellulose membrane ELISA with antisera specific for SPCSV obtained from the International Potato Center. Total RNA was extracted from young leaves of sweet potato and reverse transcription-PCR was performed by using primer pairs SPSP1 (5'-ATGRMTACTGRCAAAGTAAACGATG-3') and SPSP4 (5'-TCAACAGTGAAGACCRGYACCRGTCAA-3') corresponding to the capsid protein (CP) gene of SPCSV. Expected DNA fragments of 774 bp were obtained from symptomatic plants but not from control plants. The 774-bp fragments obtained by amplification were purified and cloned into the PMD19-T vector (TaKaRa, Dalian, China). Recombinant plasmids were then transformed into competent cells of Escherichia coli strain Jm109. Sequencing of the fragments from two individually clones plasmids yielded a nucleotide sequence (GenBank Accession No. HM773432) with 97.4 to 98.7% similarity to the CP gene of East African SPCSV isolates (1). To our knowledge, this is the first report of SPCSV in sweet potato fields from China. This virus may threaten sweet potato production in China, so cultivars and germplasm should be evaluated for resistance. References: (1) V. Aritua et al. J. Phytopathol. 156:181, 2008. (2) R. W. Njeru et al. Ann. Appl. Biol. 145:71, 2004. (3) G. A. Schaefer and E. R. Terry. Phytopathology 66:642, 1976.
甘薯褪绿矮化病毒（SPCSV）（隶属毛形病毒属，长线形病毒科）是一种由粉虱传播的、可引发甘薯病毒病（SPVD）的共侵染因子，它会给甘薯（Ipomoea batatas (L.) Lam.）造成严重的产量损失（2,3）。2009年8月在广东省从表现出SPVD典型症状（包括植株矮化、叶片扭曲、叶脉失绿和褪绿）的广薯87甘薯植株中分离出一个分离株。通过用从国际马铃薯中心获得的针对SPCSV的抗血清进行硝酸纤维素膜酶联免疫吸附测定（ELISA），对有症状植株的叶片提取物检测显示SPCSV呈阳性。从甘薯幼叶中提取总RNA，并使用与SPCSV衣壳蛋白（CP）基因对应的引物对SPSP1（5'-ATGRMTACTGRCAAAGTAAACGATG-3'）和SPSP4（5'-TCAACAGTGAAGACCRGYACCRGTCAA-3'）进行逆转录聚合酶链反应（RT-PCR）。从有症状植株中获得了预期的774 bp DNA片段，而对照植株中未获得。对扩增得到的774 bp片段进行纯化，并克隆到PMD19-T载体（宝生物工程（大连）有限公司，中国大连）中。然后将重组质粒转化到大肠杆菌JM109感受态细胞中。对两个单独克隆的质粒片段进行测序，得到一个核苷酸序列（GenBank登录号：HM773432），与东非SPCSV分离株的CP基因有97.4%至98.7%的相似性（1）。据我们所知，这是中国甘薯田首次报道SPCSV。这种病毒可能会威胁中国的甘薯生产，因此应对品种和种质进行抗性评估。参考文献：（1）V. Aritua等人，《植物病理学杂志》156:181，2008年。（2）R. W. Njeru等人，《应用生物学年鉴》145:71，2004年。（3）G. A. Schaefer和E. R. Terry，《植物病理学》66:642，1976年。
