Engel E A, Rivera P A, Valenzuela P D T
Fundación Ciencia para la Vida and MIFAB, Zañartu 1482, Universidad Andrés Bello, Facultad de Ciencias Biológicas, República 217, Santiago, Chile.
Fundación Ciencia para la Vida and MIFAB, Zañartu 1482, Santiago, Chile.
Plant Dis. 2010 May;94(5):633. doi: 10.1094/PDIS-94-5-0633A.
At least 58 viruses have been reported to infect grapevines, causing economic damage globally. Our lab has reported previously the presence of more than 10 viral species in Chilean grapevines (2,3). Grapevine Syrah virus-1 (GSyV-1) is a novel marafivirus recently described in California vineyards (1). Grapevine virus Q (GVQ) was described shortly after GSyV-1 and both genomes share more than 99% nucleotide identity (4). Since GSyV-1 and GVQ correspond to the same viral species, the name GSyV-1 will be used in the current note to avoid confusion. Forty dormant cane samples from 12 different cultivars were collected from different regions of Chile and screened by reverse transcription-PCR. One of the 40 samples (cv. Syrah) collected from the VI region of Chile was found to be infected with GSyV-1 using two different pairs of GSyV-1-specific primers. The first pair of primers GSyV-1Det-F: 5'-CAAGCCATCCGTGCATCTGG -3' and GSyV-1Det-R: 5'-GCCGATTTGGAACCCGATGG -3' (1), was used to amplify a 297-bp fragment corresponding to a partial region of the putative methyltransferase gene. The sequence (GenBank Accession No. GU566025) shared 87% nucleotide and 100% amino acid identities with the corresponding fragment of a Californian GSyV-1 isolate (GenBank Accession No. FJ436028). Since there are no commercial antibodies available for GSyV-1 detection, a second pair of primers, GVQCP-F: 5'-TCCCAGCTTCAGGGTGAATT -3' and GVQCP-R: 5'-GCATTGCTGCGCATTGGAGG -3' (4), that amplified a 720-bp fragment of the putative coat protein gene was also used. The sequence of 720 bp from the Chilean sample (GenBank Accession No. GU566024) shared 92% nucleotide and 98% amino acid identities with the corresponding fragment of a Californian GSyV-1 isolate (GenBank Accession No. FJ436028). The GSyV-1-positive sample was also infected with Grapevine fleck virus and Grapevine rupestris stem pitting-associated virus that have been reported previously in Chile. To our knowledge, this is the first report of GSyV-1 in Chile. Further studies will help to establish the incidence and effects of this virus in Chilean grapevines. References: (1) M. Al Rwahnih et al. Virology 387:395, 2009. (2) E. Engel et al. J. Virol. Methods. 163:445, 2010. (3) P. F. Escobar et al. Plant Dis. 92:1474, 2008. (4) S. Sabanadzovic et al. Virology 394:1, 2009.
据报道，至少有58种病毒可感染葡萄藤，在全球范围内造成经济损失。我们实验室此前已报道智利葡萄藤中存在10多种病毒（参考文献2,3）。葡萄西拉病毒1（GSyV-1）是一种新型马拉病毒，最近在加利福尼亚葡萄园被发现（参考文献1）。葡萄病毒Q（GVQ）在GSyV-1之后不久被描述，两者基因组的核苷酸同一性超过99%（参考文献4）。由于GSyV-1和GVQ属于同一病毒种类，本说明将使用GSyV-1这个名称以避免混淆。从智利不同地区收集了来自12个不同品种的40个休眠藤条样本，并通过逆转录PCR进行筛选。使用两对不同的GSyV-1特异性引物，发现从智利第六大区收集的40个样本中的一个（西拉品种）感染了GSyV-1。第一对引物GSyV-1Det-F：5'-CAAGCCATCCGTGCATCTGG -3'和GSyV-1Det-R：5'-GCCGATTTGGAACCCGATGG -3'（参考文献1），用于扩增对应假定甲基转移酶基因部分区域的297 bp片段。该序列（GenBank登录号GU566025）与加利福尼亚GSyV-1分离株的相应片段（GenBank登录号FJ436028）的核苷酸同一性为87%，氨基酸同一性为100%。由于没有可用于检测GSyV-1的商业抗体，还使用了另一对引物GVQCP-F：5'-TCCCAGCTTCAGGGTGAATT -3'和GVQCP-R：5'-GCATTGCTGCGCATTGGAGG -3'（参考文献4），该引物扩增假定衣壳蛋白基因的720 bp片段。来自智利样本的720 bp序列（GenBank登录号GU566024）与加利福尼亚GSyV-1分离株的相应片段（GenBank登录号FJ436028）的核苷酸同一性为92%，氨基酸同一性为98%。GSyV-1阳性样本还感染了葡萄斑点病毒和葡萄扇叶病毒，此前在智利已有相关报道。据我们所知，这是GSyV-1在智利的首次报道。进一步的研究将有助于确定该病毒在智利葡萄藤中的发生率和影响。参考文献：（1）M. Al Rwahnih等人，《病毒学》387:395，2009年。（2）E. Engel等人，《病毒学方法杂志》163:445，2010年。（3）P. F. Escobar等人，《植物病害》92:1474，2008年。（4）S. Sabanadzovic等人，《病毒学》394:1，2009年。
