源自香蕉束顶病毒DNA - 1至 - 5的启动子在转基因香蕉（芭蕉属物种）中指导维管束相关表达。

Promoters derived from banana bunchy top virus DNA-1 to -5 direct vascular-associated expression in transgenic banana (Musa spp.).

作者信息

Dugdale B, Becker D K, Beetham P R, Harding R M, Dale J L

机构信息

Centre for Molecular Biotechnology, School of Life Sciences, Queensland University of Technology, GPO Box 2434, Brisbane, Queensland, Australia 4001 e-mail:

出版信息

Plant Cell Rep. 2000 Jul;19(8):810-814. doi: 10.1007/s002999900185.

DOI:10.1007/s002999900185

PMID:30754874

Abstract

The intergenic regions of banana bunchy top virus (BBTV) DNA-1 to -5 were fused to the green fluorescent protein (GFP) and uidA reporter genes and assessed for promoter activity in transgenic banana (Musa spp. cv. Bluggoe). Promoter activity associated with the BBTV-derived promoters was transgene dependent with greatest activity observed using the GFP reporter. The BBTV promoters (BT1 to BT5) directed expression primarily in vascular-associated cells, although levels of activity varied between individual promoters. Promoters BT4 and BT5 directed the highest levels of GFP expression, while activity from BT1, BT2 and BT3 promoters was considerably weaker. Intron-mediated enhancement, using the maize polyubiquitin 1 (ubi1) intron, generated a significant increase in GUS expression directed by the BBTV promoters in transgenic plants.

摘要

将香蕉束顶病毒（BBTV）DNA-1至-5的基因间隔区与绿色荧光蛋白（GFP）和uidA报告基因融合，并在转基因香蕉（Musa spp. cv. Bluggoe）中评估其启动子活性。与源自BBTV的启动子相关的启动子活性是转基因依赖性的，使用GFP报告基因时观察到的活性最高。尽管各个启动子的活性水平有所不同，但BBTV启动子（BT1至BT5）主要在与维管束相关的细胞中指导表达。启动子BT4和BT5指导的GFP表达水平最高，而BT1、BT2和BT3启动子的活性则弱得多。使用玉米多聚泛素1（ubi1）内含子进行内含子介导的增强作用，使转基因植物中由BBTV启动子指导的GUS表达显著增加。