基于二维 SERS 编码的高通量生物分析中珠上多肽测序方法。

Two-dimensional SERS encoding method for on-bead peptide sequencing in high-throughput bioanalysis.

机构信息

Department of Chemistry Education, Seoul National University, Seoul, 08826, Republic of Korea.

出版信息

Chem Commun (Camb). 2019 Feb 26;55(18):2700-2703. doi: 10.1039/c8cc10224d.

Abstract

We developed a ready-to-read on-bead peptide encoding method for high-throughput screening bioassays. With two-dimensional surface-enhanced Raman scattering nano-identifiers (2D-SERS IDs) which are concurrently labelled with two SERS codes (coupling steps and kinds of amino acid), we could possibly generate more than 10 trillion codes with only 30 Raman label compounds.

摘要

我们开发了一种适用于高通量筛选生物测定的即用型珠上肽编码方法。利用二维表面增强拉曼散射纳米标识符（2D-SERS IDs），同时标记两个 SERS 码（偶联步骤和氨基酸种类），我们仅用 30 种拉曼标记化合物就有可能生成超过 10 万亿种编码。