Department of Pathophysiology, Anhui Medical University, Hefei, China.
Department of Obstetrics and Gynecology, Reproductive Medicine Center, Anhui Province Key Laboratory of Reproductive Health and Genetics, Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, The First Affiliated Hospital of Anhui Medical University, Anhui Medical University, Hefei, China.
Am J Reprod Immunol. 2019 Apr;81(4):e13101. doi: 10.1111/aji.13101. Epub 2019 Mar 7.
The function of CD49a on human decidual natural killer (dNK) cells is unknown.
The expression of CD49a on dNK cells from human patients with recurrent spontaneous abortions or age-matched healthy controls was analyzed by flow cytometry. DNK cells were treated with CD49a neutralizing antibody and analyzed for function (cytokines production and cytotoxic activity). Long non-coding RNA (lncRNA) microarray analysis was used to identify a potential regulator of CD49a.
DNK cells from human patients who underwent recurrent spontaneous abortions had lower levels of CD49a and increased perforin, granzyme B, and IFN-r expression, when compared to dNK cells from age-matched healthy controls. Perforin, granzyme B, and IFN-r expression levels in dNK cells were upregulated, while the migration and adhesion of dNK cells were downregulated by CD49a-neutralizing antibody. By the Cr release assay, the killing activity of dNK cells also increased with CD49a neutralizing antibody. Further, lnc-49a, a newly identified lncRNA, was shown to be a positive regulator of CD49a in primary human NK cells.
CD49a is involved in the regulation of dNK cells functions, including cytotoxic activity, migration, and adhesion. Further, lnc-49a is a positive regulator of CD49a in human primary dNK cells.
CD49a 在人蜕膜自然杀伤 (dNK) 细胞中的功能未知。
通过流式细胞术分析复发性自然流产患者或年龄匹配的健康对照者蜕膜自然杀伤 (dNK) 细胞中 CD49a 的表达。用 CD49a 中和抗体处理 dNK 细胞并分析其功能（细胞因子产生和细胞毒性活性）。使用长链非编码 RNA (lncRNA) 微阵列分析鉴定 CD49a 的潜在调节剂。
与年龄匹配的健康对照者的 dNK 细胞相比，复发性自然流产患者的 dNK 细胞中 CD49a 水平较低，穿孔素、颗粒酶 B 和 IFN-r 的表达增加。CD49a 中和抗体上调 dNK 细胞中穿孔素、颗粒酶 B 和 IFN-r 的表达水平，同时下调 dNK 细胞的迁移和黏附。通过 Cr 释放测定，dNK 细胞的杀伤活性也随着 CD49a 中和抗体的增加而增加。此外，新鉴定的 lncRNA lnc-49a 被证明是人类原代 NK 细胞中 CD49a 的正向调节剂。
CD49a 参与调节 dNK 细胞的功能，包括细胞毒性活性、迁移和黏附。此外，lnc-49a 是人类原代 dNK 细胞中 CD49a 的正向调节剂。
