Zhang Y J, Li G F, Li M F
Chinese Academy of Inspection and Quarantine, Beijing 100029, China.
Plant Dis. 2009 Feb;93(2):200. doi: 10.1094/PDIS-93-2-0200C.
Cucumber green mottle mosaic virus (CGMMV) was first discovered in 2003 in China (2) and developed an epidemic during 2005 (1). To know the occurrence scale and damage level of CGMMV, a survey was carried out in the main areas of cucurbitaceous plant production and seed trading in 2006 and 2007 in Mainland China. Samples of 739 plants of 16 types (seed samples from Cucurbita moschata, Cucumis melo, Lagenaria siceraria, Cucumis sativus, Momordica charantia, L. siceraria var. clavata, and C. pepo, leaf samples from C. moschata, Cucumis melo, L. siceraria, Cucumis sativus, and M. charantia, fruit samples from C. moschata, Citrullus lanatus, and L. siceraria, and seedling samples from Citrullus lanatus) were collected from 13 regions and analyzed by a double antibody sandwich (DAS)-ELISA. CGMMV was detected in Citrullus lanatus, L. siceraria, Cucumis melo, and C. moschata from six regions in 2006. Citrullus vulgaris, Cucumis melo, and C. moschata were infected most frequently; in 76, 60, and 30 of the leaf samples, respectively. CGMMV was tested positive in the samples of 8 seedlings, 23 seeds, and 1 fruit. In contrast, all samples tested negative in 2007. To confirm CGMMV identification, ELISA-positive samples were detected by reverse transcription-PCR assay using virus-specific primers that amplified a 524-bp fragment in the coat protein coding region. The nucleotide sequence of the PCR product (GenBank Accession No. DQ997778) isolated form L. siceraria in Liaoning Province (LHP) showed 100% identity with the Japanese watermelon strain (Japan W) and 91% identity with the Greece GR5 strain. On the basis of the symptoms of indicator plants (chlorotic spot and systemic mosaic were observed in L. siceraria, Chenopodium amaranticolor, and Cucumis sativus; no infection was observed in Datura stramonium, Nicotiana tabacum, and Chenopodium quinoa) and coat protein nucleotide sequence comparisons, the two CGMMV isolates of Wcn (1) and LHP from China should be grouped into the watermelon strain. The results showed that the government should establish effective quarantine strategy and the growers take proper planting measures to avoid further spreading of this virus. References: (1) H. Y. Chen et al. Acta Phytopathol. Sin. 36:306, 2006. (2) B. X. Qin et al. Plant Quarantine. 19:4, 2005.
黄瓜绿斑驳花叶病毒（CGMMV）于2003年首次在中国被发现（2），并在2005年爆发了疫情（1）。为了解CGMMV的发生规模和危害程度，于2006年和2007年在中国内地主要的葫芦科植物种植区和种子交易区开展了一项调查。从13个地区采集了16种类型的739株植物样本（包括南瓜、甜瓜、瓠瓜、黄瓜、苦瓜、瓠瓜变种、西葫芦的种子样本，南瓜、甜瓜、瓠瓜、黄瓜、苦瓜的叶片样本，南瓜、西瓜、瓠瓜的果实样本，西瓜的幼苗样本），并通过双抗体夹心（DAS）-ELISA进行分析。2006年在6个地区的西瓜、瓠瓜、甜瓜和南瓜中检测到了CGMMV。普通西瓜、甜瓜和南瓜的感染最为频繁；分别在76份、60份和30份叶片样本中检测到。在8株幼苗、23粒种子和1个果实的样本中，CGMMV检测呈阳性。相比之下，2007年所有样本检测均为阴性。为确认CGMMV的鉴定结果，使用病毒特异性引物通过逆转录-PCR检测ELISA阳性样本，该引物在外壳蛋白编码区扩增出一个524 bp的片段。从辽宁省瓠瓜中分离得到的PCR产物（GenBank登录号：DQ997778）的核苷酸序列与日本西瓜株系（Japan W）的同源性为100%，与希腊GR5株系的同源性为91%。根据指示植物的症状（在瓠瓜、苋色藜和黄瓜中观察到褪绿斑点和系统花叶；在曼陀罗、烟草和藜麦中未观察到感染）以及外壳蛋白核苷酸序列比较，来自中国的Wcn（1）和LHP这两个CGMMV分离株应归为西瓜株系。结果表明，政府应制定有效的检疫策略，种植者应采取适当的种植措施，以避免这种病毒的进一步传播。参考文献：（1）陈洪运等人，《植物病理学报》36:306，2006年。（2）秦碧霞等人，《植物检疫》19:4，2005年。
