关于甘蔗锈病（由古巴柄锈菌引起）在哥斯达黎加和尼加拉瓜的首次报道。

First Report of Orange Rust of Sugarcane Caused by Puccinia kuehnii in Costa Rica and Nicaragua.

作者信息

Chavarría E, Subirós F, Vega J, Ralda G, Glynn N C, Comstock J C, Castlebury L A

机构信息

Liga Agricola Industrial de la Caña de Azucar, San Josė, Costa Rica.

Azucarera El Viejo, S. A., Guanacaste, Costa Rica.

出版信息

Plant Dis. 2009 Apr;93(4):425. doi: 10.1094/PDIS-93-4-0425C.

DOI:10.1094/PDIS-93-4-0425C

PMID:30764243

Abstract

Symptoms and signs of orange rust on sugarcane (a complex hybrid of Saccharum L. species) were observed from July 2007 on cv. SP 71-5574 in Costa Rica at the Coopeagri Sugar Mill located in Pérez Zeledón, San José and on multiple cultivars (CP 72-2086, Pindar, Q 132, Q 138, SP 71-5574, and SP 79-2233) at the Providencia Sugar Mill near Muelle, San Carlos and Cutris Sugar Mill near Los Chiles during August 2007. The same symptoms and signs were observed on cv. CP 72-2086 during September 2007 in Nicaragua at Ingenio San Antonio, located near Chinandega, and Ingenio Monte Rosa near El Viejo, Nicaragua. Disease symptoms and uredinia appeared different from brown rust caused by Puccinia melanocephala, and brown rust usually does not occur on these cultivars. Uredinia and urediniospores were similar to those described for orange rust (1,2). Cvs. SP 71-5574 and SP 79-2233 are susceptible and cv. CP 72-2086 is moderately susceptible to orange rust in Costa Rica and cvs. ISACP 00-1075, ISA 00-1000, and CP 72-2086 are moderately susceptible in Nicaragua. Samples from both locations (Costa Rica BPI No. 878816 and Nicaragua BPI No. 878817) examined at the USDA-ARS Mycology and Microbiology Laboratory in Beltsville, MD showed morphological characteristics consistent with those of P. kuehnii. Analysis of ITS1, 5.8S, and ITS2 rDNA sequences of the rust infecting cv. CP 72-2086 (GenBank Accession No. FJ532477) from Costa Rica and cv. ISA 00-1000 from Nicaragua (GenBank Accession No. FJ532476) confirmed the identity as P. kuehnii, the causal agent of sugarcane orange rust. Beside the cultivars already mentioned, orange rust also was confirmed on cvs. RB 73-9735 and CPCL 02-2130 in Costa Rica. To our knowledge, this is the first report of orange rust of sugarcane in Costa Rica and Nicaragua and the third confirmation of the disease in the Western Hemisphere and Caribbean Basin. References: (1) J. C. Comstock et al. Plant Dis. 92:175, 2008. (2) W. Ovalle et al. Plant Dis. 92:973, 2008.

摘要

2007年7月起，在哥斯达黎加位于圣何塞省佩雷斯·塞莱东的Coopeagri糖厂，观察到甘蔗（甘蔗属物种的复杂杂交种）上橙锈病的症状和体征，该甘蔗品种为SP 71 - 5574。2007年8月，在圣卡洛斯省穆埃尔附近的普罗维登西亚糖厂以及洛斯奇莱斯附近的库特里斯糖厂，在多个品种（CP 72 - 2086、品达、Q 132、Q 138、SP 71 - 5574和SP 79 - 2233）上也观察到了相同症状和体征。2007年9月，在尼加拉瓜位于奇南德加附近的圣安东尼奥糖厂以及尼加拉瓜旧韦霍附近的蒙特罗萨糖厂，在CP 72 - 2086品种上观察到了相同症状和体征。病害症状和夏孢子堆与黑顶柄锈菌引起的褐锈病不同，而且这些品种通常不会发生褐锈病。夏孢子堆和夏孢子与已描述的橙锈病的特征相似。在哥斯达黎加，SP 71 - 5574和SP 79 - 2233品种对橙锈病敏感，CP 72 - 2086品种中度敏感；在尼加拉瓜，ISACP 00 - 1075、ISA 00 - 1000和CP 72 - 2086品种中度敏感。从两个地点采集的样本（哥斯达黎加BPI编号878816和尼加拉瓜BPI编号878817）在美国农业部农业研究局位于马里兰州贝尔茨维尔的真菌学和微生物学实验室进行检测，结果显示其形态特征与昆氏柄锈菌一致。对来自哥斯达黎加感染CP 72 - 2086品种（GenBank登录号FJ532477）和尼加拉瓜ISA 00 - 1000品种（GenBank登录号FJ532476）的锈菌的ITS1、5.8S和ITS2 rDNA序列进行分析，确认其为昆氏柄锈菌，即甘蔗橙锈病的病原菌。除了上述已提及的品种外，在哥斯达黎加的RB 73 - 9735和CPCL 02 - 2130品种上也确认发现了橙锈病。据我们所知，这是哥斯达黎加和尼加拉瓜首次报道甘蔗橙锈病，也是西半球和加勒比海地区第三次确认该病害。参考文献：(1) J. C. Comstock等人，《植物病害》92:175，2008年。(2) W. Ovalle等人，《植物病害》92:973，2008年。