Wang Jinxin, DePena Mara, Taylor Graham, Gilbert Elizabeth R, Cline Mark A
Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States.
School of Neuroscience, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States.
Gen Comp Endocrinol. 2019 May 15;276:22-29. doi: 10.1016/j.ygcen.2019.02.015. Epub 2019 Feb 12.
Central administration of corticotropin-releasing factor (CRF), a 41-amino acid peptide, is associated with anorexigenic effects across various species, with particularly potent reductions in food intake in rodents and chickens (Gallus gallus domesticus), a species for which the most is known. The purpose of the current study was to determine the hypothalamic mechanism of CRF-induced anorexigenic effects in 7 day-old Japanese quail (Coturnix japonica), a less-intensely-selected gallinaceous relative to the chicken that can provide more evolutionary perspective. After intracerebroventricular (ICV) injection of 2, 22, or 222 pmol of CRF, a dose-dependent decrease in food intake was observed that lasted for 3 and 24 h for the 22 and 222 pmol doses, respectively. The 2 pmol dose had no effect on food or water intake. The numbers of c-Fos immunoreactive cells were increased in the paraventricular nucleus (PVN) and lateral hypothalamic area (LHA) at 1 h post-injection in quail injected with 22 pmol of CRF. The hypothalamic mRNA abundance of proopiomelanocortin, melanocortin receptor subtype 4, CRF, and CRF receptor sub-type 2 was increased at 1 h in quail treated with 22 pmol of CRF. Behavior analyses demonstrated that CRF injection reduced feeding pecks and jumps and increased the time spent standing. In conclusion, results demonstrate that the anorexigenic effects of CRF in Japanese quail are likely influenced by the interaction between CRF and melanocortin systems and that injection of CRF results in species-specific behavioral changes.
促肾上腺皮质激素释放因子（CRF）是一种由41个氨基酸组成的肽，对多种物种都有抑制食欲的作用，在啮齿动物和鸡（家鸡）中能特别有效地减少食物摄入量，鸡是人们了解最多的物种。本研究的目的是确定CRF诱导7日龄日本鹌鹑（日本鹌鹑）产生厌食作用的下丘脑机制，相对于鸡而言，日本鹌鹑是一种经过较少强化选择的鸡形目动物，能提供更多的进化视角。脑室内（ICV）注射2、22或222 pmol的CRF后，观察到食物摄入量呈剂量依赖性下降，22和222 pmol剂量分别持续3小时和24小时。2 pmol剂量对食物或水的摄入量没有影响。在注射22 pmol CRF的鹌鹑中，注射后1小时，室旁核（PVN）和下丘脑外侧区（LHA）中c-Fos免疫反应性细胞的数量增加。在接受22 pmol CRF治疗的鹌鹑中，1小时时促阿片黑素皮质素、黑皮质素受体亚型4、CRF和CRF受体亚型2的下丘脑mRNA丰度增加。行为分析表明，注射CRF减少了啄食和跳跃行为，并增加了站立时间。总之，结果表明，CRF在日本鹌鹑中的厌食作用可能受CRF和黑皮质素系统之间相互作用的影响，并且注射CRF会导致物种特异性行为变化。
