Tajbakhsh Amir, Ghasemi Faezeh, Mirbagheri Seyedeh Zohre, Momen Heravi Mastoureh, Rezaee Mehdi, Meshkat Zahra
Dept. of New Sciences and Technologies, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Pathol. 2018 Fall;13(4):429-437. Epub 2018 Sep 25.
The incidence of rifampin-resistant strains of has attracted more attention than the tuberculosis infection due to laborious treatment and control. Recognizing the genotypes involving in drug resistance via multiplex PCR, a simple and rapid genotyping method, is an emergency for better treatment and control of tuberculosis. This study was designed to specify the frequency of rifampin-resistant strains of isolated from patients by multiplex allele-specific Polymerase Chain Reaction assay (MAS-PCR).
In this study, 88 positive samples were included from Qaem Hospital, Mashhad. MAS-PCR was used to detect the rifampin resistance associated mutations in gene.
Mutations in three codons of gene causing rifampin resistance were detected in 51 isolates (58.96%). The detected mutations in codons 531, 526, and 516 were 55.68%, 38.63%, and 13.63%, respectively. The simultaneous mutations were detected in 11 isolates (12.50%) in codons 531, 526 and 516, in 21 isolates (23.86%) in codons 531 and 526, and in one isolate (1.13%) in codons 526 and 516.
According to the results of this study, the frequency of rifampin-resistant strains of isolated from Khorasan province patients (North-East of Iran) was high. The developed MAS-PCR assay can be used for rapid detection in clinical diagnostic laboratories in areas with high prevalence of multidrug-resistant strains. In this respect, MAS-PCR is simple, rapid, and highly sensitive method for drug susceptibility tests for detecting multidrug-resistant .
由于治疗和控制工作艰巨，耐利福平菌株的发生率比结核病感染更受关注。通过多重聚合酶链反应（一种简单快速的基因分型方法）识别涉及耐药性的基因型，是更好地治疗和控制结核病的当务之急。本研究旨在通过多重等位基因特异性聚合酶链反应分析（MAS-PCR）确定从患者中分离出的耐利福平菌株的频率。
本研究纳入了来自马什哈德卡姆医院的88份阳性样本。采用MAS-PCR检测基因中与利福平耐药相关的突变。
在51株分离株（58.96%）中检测到基因三个密码子的突变导致利福平耐药。密码子531、526和516的检测突变分别为55.68%、38.63%和13.63%。在密码子531、526和516的11株分离株（12.50%）、密码子531和526的21株分离株（23.86%）以及密码子526和516的1株分离株（1.13%）中检测到同时突变。
根据本研究结果，从霍拉桑省患者（伊朗东北部）分离出的耐利福平菌株频率较高。所开发的MAS-PCR检测方法可用于多药耐药菌株高流行地区的临床诊断实验室进行快速检测。在这方面，MAS-PCR是一种简单、快速且高度灵敏的检测多药耐药的药敏试验方法。
