聚焦蓝藻纲（红藻门）中的Rad52：藻类遗传中的遗迹

A Spotlight on Rad52 in Cyanidiophytina (Rhodophyta): A Relic in Algal Heritage.

作者信息

Del Mondo Angelo, Iovinella Manuela, Petriccione Milena, Nunziata Angelina, Davis Seth J, Cioppa Diana, Ciniglia Claudia

机构信息

Department of Biology, University of Naples Federico II, Via Cinthia 21, 80126 Naples, Italy.

Department of Biology, University of York, York YO105DD, UK.

出版信息

Plants (Basel). 2019 Feb 19;8(2):46. doi: 10.3390/plants8020046.

DOI:10.3390/plants8020046

PMID:30791384

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6410040/

Abstract

The RADiation sensitive52 (RAD52) protein catalyzes the pairing between two homologous DNA sequences' double-strand break repair and meiotic recombination, mediating RAD51 loading onto single-stranded DNA ends, and initiating homologous recombination and catalyzing DNA annealing. This article reports the characterization of RAD52 homologs in the thermo-acidophilic Cyanidiophyceae whose genomes have undergone extensive sequencing. Database mining, phylogenetic inference, prediction of protein structure and evaluation of gene expression were performed in order to determine the functionality of the RAD52 protein in Cyanidiophyceae. Its current function in Cyanidiophytina could be related to stress damage response for thriving in hot and acidic environments as well as to the genetic variability of these algae, in which, conversely to extant Rhodophyta, sexual mating was never observed.

摘要

辐射敏感蛋白52（RAD52）催化两个同源DNA序列之间的双链断裂修复和减数分裂重组配对，介导RAD51加载到单链DNA末端，并启动同源重组和催化DNA退火。本文报道了嗜热嗜酸蓝藻纲中RAD52同源物的特征，该纲的基因组已进行了广泛测序。为了确定RAD52蛋白在蓝藻纲中的功能，进行了数据库挖掘、系统发育推断、蛋白质结构预测和基因表达评估。它目前在蓝藻门中的功能可能与在高温和酸性环境中生存的应激损伤反应以及这些藻类的遗传变异性有关，与现存红藻相反，在这些藻类中从未观察到有性交配。