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盐酸小檗碱对 和作用的抑制及其机制。

Inhibitory Effects of Berberine Hydrochloride on and the Underlying Mechanisms.

机构信息

Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

出版信息

Molecules. 2019 Feb 19;24(4):742. doi: 10.3390/molecules24040742.

DOI:10.3390/molecules24040742
PMID:30791402
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6412246/
Abstract

BACKGROUND

can infect all mammals, including rabbits, causing serious infections with remarkable economic losses for rabbit farmers. Berberine is an alkaloid that is effective against a variety of microbial infections such as . Growth curve by dry weight determination and in-vivo antifungal assay were carried out to clarify the inhibitory effect of berberine hydrochloride against . Transcriptomics analyses were also carried out for better understanding of the underlying mechanisms.

RESULTS

The growth rate of was significantly higher in control condition than under berberine hydrochloride or clotrimazole for 60 h. The growth rate of was significantly slighter higher in berberine condition (1 mg) than under clotrimazole for 46 h. seriously shrunk after berberine or clotrimazole treatment, as observed by TEM and in SEM. Significant recovery was evident in three berberine groups on day 6 compared with the DMSO group. Results from transcriptomics analyses showed 18,881 identified unigenes, including 18,754 and 12,127 in the NT and SwissProt databases. Among these, 12,011, 9174, and 11,679 unigenes belonged to 3 Gene Ontology (GO), 43 KEGG, and 25 KOG categories, respectively. Interestingly, we found that down-regulation of 14α-demethylase exposed to various medicines was slightly different, i.e., berberine hydrochloride (fold change -3.4956) and clotrimazole (fold change -2.1283) caused various degrees of alteration.

CONCLUSIONS

Berberine hydrochloride could inhibit the growth of . Berberine hydrochloride could also cure dermatosis induced by . Down-regulation of 14α-demethylase exposed to various medicines was slightly different and might be one of the anti-resistance mechanisms of berberine hydrochloride in . The present investigation provides considerable transcript sequence data that would help further assess the antifungal mechanisms against , for antifungal medicine development.

摘要

背景

可感染包括兔子在内的所有哺乳动物，导致兔农遭受严重感染和显著的经济损失。小檗碱是一种生物碱，对多种微生物感染有效，如 。通过干重测定和体内抗真菌试验进行生长曲线研究，以阐明盐酸小檗碱对 的抑制作用。还进行了转录组学分析，以更好地了解潜在机制。

结果

在对照条件下， 的生长速度明显高于盐酸小檗碱或克霉唑 60 小时。在 1 毫克盐酸小檗碱条件下， 的生长速度明显高于克霉唑 46 小时。经盐酸小檗碱或克霉唑处理后， 严重收缩，TEM 和 SEM 观察到这一点。与 DMSO 组相比，在第 6 天，三个盐酸小檗碱组均有明显恢复。转录组学分析结果显示，共鉴定出 18881 个基因，其中 NT 和 SwissProt 数据库分别有 18754 个和 12127 个。在这些基因中，有 12011 个、9174 个和 11679 个基因分别属于 3 个基因本体论（GO）、43 个京都基因与基因组百科全书（KEGG）和 25 个同源基因（COG）类别。有趣的是，我们发现，暴露于各种药物的 14α-脱甲基酶的下调略有不同，即盐酸小檗碱（倍数变化-3.4956）和克霉唑（倍数变化-2.1283）导致了不同程度的改变。

结论

盐酸小檗碱能抑制 的生长。盐酸小檗碱还能治愈 引起的皮肤病。暴露于各种药物的 14α-脱甲基酶的下调略有不同，这可能是盐酸小檗碱在 中抗耐药的机制之一。本研究提供了大量的转录序列数据，有助于进一步评估针对 的抗真菌机制，为抗真菌药物的开发提供参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/a73ff73338bc/molecules-24-00742-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/188099742f23/molecules-24-00742-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/e9f7ac300c5d/molecules-24-00742-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/5e9e45b3be26/molecules-24-00742-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/87f51ab43df3/molecules-24-00742-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/047793f1bef6/molecules-24-00742-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/10f9ea2dfff9/molecules-24-00742-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/85fd4dae240a/molecules-24-00742-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/4411523b1c47/molecules-24-00742-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/281796223ac9/molecules-24-00742-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/a73ff73338bc/molecules-24-00742-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/188099742f23/molecules-24-00742-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/e9f7ac300c5d/molecules-24-00742-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/5e9e45b3be26/molecules-24-00742-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/87f51ab43df3/molecules-24-00742-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/047793f1bef6/molecules-24-00742-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/10f9ea2dfff9/molecules-24-00742-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/85fd4dae240a/molecules-24-00742-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/4411523b1c47/molecules-24-00742-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/281796223ac9/molecules-24-00742-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe1e/6412246/a73ff73338bc/molecules-24-00742-g010.jpg

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