a Medical Microbiology and Immunology Department, Faculty of Medicine , Mansoura University , Mansoura , Egypt.
b Obstetrics and Gynecology Department, Faculty of Medicine , Mansoura University , Mansoura , Egypt.
Immunol Invest. 2019 Aug;48(6):608-617. doi: 10.1080/08820139.2019.1578790. Epub 2019 Feb 22.
: This study was conducted to compare the levels of maternal serum soluble endoglin (sEng) and cell-free fetal DNA (cffDNA) in pregnant females with PE to normotensive pregnant ones, together with relating these levels to preeclampsia (PE) severity and onset. : It was a comparative study in Mansoura University Hospital, Egypt, to detect the levels of serum sEng by ELISA besides the levels of cffDNA by quantitative real-time polymerase chain reaction in 80 pregnant females suffering from PE in addition to 80 normotensive pregnant ones that were included as control. : Levels of serum sEng and cffDNA were higher in PE cases than control (p < 0.0001٭ both) and were significantly related to the severity of the disease. Levels were also higher in early than late onset PE (p < 0.003٭ and <0.002٭, respectively). Sensitivities, specificities, positive, and negative predictive values in addition to accuracy of serum sEng and cffDNA were 97.5%, 98.8%, 98.7%, 97.5%, and 98.1% and 97.5%, 93.8%, 94.0%, 97.4%, and 95.6%, respectively. : Maternal serum sEng and cffDNA can be good markers for diagnosis of PE in Egyptian patients. They are positively related to the disease severity. :
本研究旨在比较患有 PE 的孕妇与正常血压孕妇的血清可溶性内皮因子（sEng）和游离胎儿 DNA（cffDNA）水平，并将这些水平与子痫前期（PE）的严重程度和发病时间相关联。这是埃及曼苏拉大学医院的一项对比研究，通过 ELISA 检测血清 sEng 水平，通过实时定量聚合酶链反应（PCR）检测 cffDNA 水平，共纳入 80 例患有 PE 的孕妇和 80 例正常血压孕妇作为对照组。PE 组的血清 sEng 和 cffDNA 水平均高于对照组（p<0.0001，均为），与疾病的严重程度显著相关。早发型 PE 的水平也高于晚发型 PE（p<0.003 和 <0.002，分别）。血清 sEng 和 cffDNA 的灵敏度、特异性、阳性预测值、阴性预测值和准确性分别为 97.5%、98.8%、98.7%、97.5%和 98.1%和 97.5%、93.8%、94.0%、97.4%和 95.6%。血清可溶性内皮因子（sEng）和游离胎儿 DNA（cffDNA）可作为埃及患者诊断 PE 的良好标志物。它们与疾病的严重程度呈正相关。
Zotero 插件