用于在动态生长模式下研究鲍曼不动杆菌生物膜形成的BioFlux™ 200微流控系统。
BioFlux™ 200 Microfluidic System to Study A. baumannii Biofilm Formation in a Dynamic Mode of Growth.
作者信息
Naudin Bertrand, Heins Anja, Pinhal Stéphane, Dé Emmanuelle, Nicol Marion
机构信息
Normandie University, Unirouen, Rouen, France.
Polymers, Biopolymers, Surfaces Laboratory, CNRS, UMR 6270, UFR des Sciences et Techniques, Mont-Saint-Aignan, France.
出版信息
Methods Mol Biol. 2019;1946:167-176. doi: 10.1007/978-1-4939-9118-1_16.
The ability of A. baumannii to develop biofilms on a wide range of surfaces can be associated to its persistence in hospital settings and the emergence of recalcitrant and chronic infections. Few compounds are available to eradicate A. baumannii biofilms, and most of them have been tested for their antibiofilm properties in static conditions. Microfluidics systems as BioFlux™ system are now available for studying A. baumannii biofilm formation in dynamic conditions. Here, we described the use of this system for studying the biofilm development of the reference strain A. baumannii ATCC 17978 in a dynamic mode. We showed how to test the activity of an antibiotic (colistin at the MIC concentration, 0.5 μg/mL) in these conditions of growth.
鲍曼不动杆菌在多种表面形成生物膜的能力，可能与其在医院环境中的持续存在以及顽固性和慢性感染的出现有关。目前几乎没有化合物可用于根除鲍曼不动杆菌生物膜，并且大多数化合物已在静态条件下测试其抗生物膜特性。诸如BioFlux™系统之类的微流控系统现在可用于研究动态条件下鲍曼不动杆菌生物膜的形成。在此，我们描述了使用该系统以动态模式研究鲍曼不动杆菌参考菌株ATCC 17978生物膜发育的情况。我们展示了在这些生长条件下如何测试抗生素（MIC浓度的黏菌素，0.5μg/mL）的活性。
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