基于 HapMap 的研究：CYP2A13 可能是不吸烟人群肺癌发生过程中的潜在关键代谢酶基因。
HapMap-based study: CYP2A13 may be a potential key metabolic enzyme gene in the carcinogenesis of lung cancer in non-smokers.
机构信息
Department of Thoracic Surgery, Shandong Cancer Hospital Affiliated to Shandong University, Shandong Academy of Medical Sciences, Jinan, China.
Department of Respiratory, Jiuquan City People's Hospital, Jiuquan, China.
出版信息
Thorac Cancer. 2019 Apr;10(4):601-606. doi: 10.1111/1759-7714.12954. Epub 2019 Feb 26.
BACKGROUND
The aim of this study was to evaluate the association between CYP2A13 polymorphisms and lung cancer susceptibility using the HapMap database.
METHODS
A case-control analysis of 532 subjects with lung cancer and 614 controls with no personal history of the disease was performed. The tag SNPs rs1645690 and rs8192789 for CYP2A13 were selected, and the genetic polymorphisms were confirmed experimentally through real-time PCR, cloning, and sequencing assay.
RESULTS
SNP frequency in this study was consistent with the HapMap Project database of Han-Chinese and lung cancer risk was associated with CYP2A13 polymorphisms in non-smokers. CYP2A13 shares a 93.5% identity with CYP2A6 in the amino acid sequence and the homologous sequences may interfere with the study of SNPs of CYP2A13.
CONCLUSIONS
CYP2A13 may be a potential key metabolic enzyme gene in the carcinogenesis of lung cancer in non-smokers. The common polymorphisms of CYP2A13 may be candidate biomarkers for lung cancer susceptibility in Han-Chinese.
背景
本研究旨在利用 HapMap 数据库评估 CYP2A13 多态性与肺癌易感性之间的关联。
方法
对 532 例肺癌患者和 614 例无疾病个人史的对照进行病例对照分析。选择 CYP2A13 的标签 SNP rs1645690 和 rs8192789，并通过实时 PCR、克隆和测序实验验证遗传多态性。
结果
本研究的 SNP 频率与 Han-Chinese 的 HapMap 项目数据库一致，肺癌风险与非吸烟者 CYP2A13 多态性相关。CYP2A13 在氨基酸序列上与 CYP2A6 有 93.5%的同源性，同源序列可能会干扰 CYP2A13 SNP 的研究。
结论
CYP2A13 可能是非吸烟者肺癌发生的潜在关键代谢酶基因。CYP2A13 的常见多态性可能是非吸烟者肺癌易感性的候选生物标志物。
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