鉴定长非编码 RNA HERC2P2 作为脑胶质瘤的肿瘤抑制因子。

Identification of long non-coding RNA HERC2P2 as a tumor suppressor in glioma.

机构信息

Tianjin Neurological Institute, Key Laboratory of Post-neurotrauma Neuro-repair and Regeneration in Central Nervous System, Ministry of Education and Tianjin City, Tianjin, China.

Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, China.

出版信息

Carcinogenesis. 2019 Aug 22;40(8):956-964. doi: 10.1093/carcin/bgz043.

DOI:10.1093/carcin/bgz043

PMID:30809632

Abstract

Long non-coding RNAs (lncRNAs) have been reported to play important roles in glioma; however, most of them promote glioma progression. We constructed a competing endogenous (ceRNA) network based on the Chinese Glioma Genome Atlas dataset, and lncRNA hect domain and RLD 2 pseudogene 2 (HERC2P2) is the core of this network. Highly connected genes in the ceRNA network classified the glioma patients into three clusters with significantly different survival rates. The expression of HERC2P2 is positively correlated with survival and negatively correlated with clinical grade. Cell colony formation, Transwell and cell scratch tests were performed to evaluate the role of HERC2P2 in glioblastoma growth. Furthermore, we overexpressed HERC2P2 in U87 cells and established a mouse intracranial glioma model to examine the function of HERC2P2 in vivo. In conclusion, we identified a lncRNA with tumor suppressor functions in glioma that could be a potential biomarker for glioma patients.

摘要

长链非编码 RNA（lncRNA）已被报道在神经胶质瘤中发挥重要作用；然而，它们中的大多数促进神经胶质瘤的进展。我们基于中国神经胶质瘤基因组图谱数据集构建了一个竞争性内源 RNA（ceRNA）网络，lncRNA hect 结构域和 RLD2 假基因 2（HERC2P2）是该网络的核心。ceRNA 网络中高度连接的基因将神经胶质瘤患者分为三组，其生存率有显著差异。HERC2P2 的表达与生存呈正相关，与临床分级呈负相关。通过细胞集落形成、Transwell 和细胞划痕试验评估了 HERC2P2 在神经母细胞瘤生长中的作用。此外，我们在 U87 细胞中转染过表达 HERC2P2，并建立了小鼠颅内神经胶质瘤模型，以体内检测 HERC2P2 的功能。总之，我们在神经胶质瘤中鉴定出一种具有肿瘤抑制功能的 lncRNA，它可能是神经胶质瘤患者的潜在生物标志物。