State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Department of Chemical Biology and, Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering and, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, P. R. China.
Chembiochem. 2019 Aug 1;20(15):1898-1905. doi: 10.1002/cbic.201900035. Epub 2019 May 22.
Over the past few decades, various DNA modification detection methods have been developed; many of the high-resolution methods are based on bisulfite treatment, which leads to DNA degradation, to a degree. Thus, novel bisulfite-free approaches have been developed in recent years and shown to be useful for epigenome analysis in otherwise difficult-to-handle, but important, DNA samples, such as hmC-seal and hmC-CATCH. Herein, an overview of advances in the development of epigenome sequencing methods for these important DNA modifications is provided.
在过去的几十年中，已经开发出了各种 DNA 修饰检测方法；许多高分辨率方法都是基于亚硫酸氢盐处理的，这会在一定程度上导致 DNA 降解。因此，近年来开发了新型的无亚硫酸盐方法，并且已被证明对于其他难以处理但重要的 DNA 样本中的表观基因组分析很有用，例如 hmC-seal 和 hmC-CATCH。本文提供了这些重要 DNA 修饰的表观基因组测序方法的开发进展概述。
