通过琼脂糖电泳测定的PHA-L4同工凝集素的金属离子含量与促有丝分裂活性之间的关系。

The relationship between metal ion content and mitogenicity of PHA-L4 isolectin as determined by agarose electrophoresis.

作者信息

Glad C, Borrebaeck C A

出版信息

J Immunol Methods. 1986 Mar 13;87(2):149-53. doi: 10.1016/0022-1759(86)90524-7.

Abstract

The lectin (PHA) from Phaseolus vulgaris is a tetrameric metalloprotein which exists as 5 different isolectins. An agarose electrophoretic assay is described that can distinguish between metallized and demetallized PHA. The assay has been employed for the analysis of 5 different preparations of the isolectin PHA-L4, and the preparations have subsequently been tested for their ability to induce lymphocyte transformation. A reverse correlation was found between the relative amount of irreversibly demetallized isolectin, as determined by agarose electrophoresis and the mitogenic activity of the preparation. The degree of irreversible demetallization seemed to be dependent on the preparation method used. One method gave 25% and another 100% demetallization of the isolectins. The assay was found to be a rapid and simple way to test the relative amount of mitogenically active lectin present in a preparation.

摘要

来自菜豆的凝集素（PHA）是一种四聚体金属蛋白，以5种不同的同工凝集素形式存在。本文描述了一种琼脂糖电泳分析法，该方法可以区分金属化和脱金属化的PHA。该分析法已用于分析同工凝集素PHA-L4的5种不同制剂，随后对这些制剂诱导淋巴细胞转化的能力进行了测试。通过琼脂糖电泳测定的不可逆脱金属化同工凝集素的相对量与制剂的促有丝分裂活性之间存在负相关。不可逆脱金属化的程度似乎取决于所使用的制备方法。一种方法使同工凝集素的脱金属化率为25%，另一种方法为100%。该分析法是测试制剂中促有丝分裂活性凝集素相对量的一种快速简便的方法。