肌醇1,4,5-三磷酸诱导的钙离子从通透化肥大细胞瘤细胞中的释放。

Inositol 1,4,5-trisphosphate-induced Ca2+ release from permeabilized mastocytoma cells.

作者信息

Muto Y, Tohmatsu T, Yoshioka S, Nozawa Y

出版信息

Biochem Biophys Res Commun. 1986 Feb 26;135(1):46-51. doi: 10.1016/0006-291x(86)90940-x.

Abstract

The effect of inositol 1,4,5-trisphosphate (IP3) on Ca2+ release in the transformed murine mast cells, mastocytoma P-815 cells permeabilized with digitonin was studied. Ca2+ was sequestered by intracellular organelles in the presence of ATP until the medium free Ca2+ concentration was lowered to a new steady-state level. The subsequent addition of IP3 caused a rapid Ca2+ release, which was followed by a slow re-uptake of Ca2+. Fifty percent of the sequestered Ca2+ was released by 10 microM IP3. Maximal Ca2+ release occurred at 10 microM and half maximal activity was at 1.3 microM. These results indicate that IP3 may function as a messenger of intracellular Ca2+ mobilization in mastocytoma cells.

摘要

研究了肌醇1,4,5 - 三磷酸（IP3）对经洋地黄皂苷通透处理的转化鼠肥大细胞——肥大细胞瘤P - 815细胞中Ca2+释放的影响。在ATP存在的情况下，Ca2+被细胞内细胞器隔离，直到细胞外游离Ca2+浓度降低到一个新的稳态水平。随后加入IP3会导致Ca2+快速释放，接着是Ca2+的缓慢再摄取。10微摩尔IP3可释放50%被隔离的Ca2+。最大Ca2+释放在10微摩尔时发生，半最大活性在1.3微摩尔时出现。这些结果表明IP3可能作为肥大细胞瘤细胞中细胞内Ca2+动员的信使发挥作用。