活化型人c-Ha-ras蛋白合成基因的合成与表达
Synthesis and expression of a synthetic gene for the activated human c-Ha-ras protein.
作者信息
Miura K, Inoue Y, Nakamori H, Iwai S, Ohtsuka E, Ikehara M, Noguchi S, Nishimura S
出版信息
Jpn J Cancer Res. 1986 Jan;77(1):45-51.
PMID:3082814
Abstract
A 576 base-pair DNA encoding human c-Ha-ras protein (p21) with a valine codon at position 12 has been synthesized by ligation of chemically synthesized oligodeoxyribonucleotides. The duplex was inserted into a plasmid with the E. coli tryptophan promoter and was expressed in E. coli efficiently.
摘要
一段编码人c-Ha-ras蛋白（p21）的576个碱基对的DNA，其第12位密码子为缬氨酸，已通过化学合成的寡脱氧核糖核苷酸连接而成。该双链体被插入到带有大肠杆菌色氨酸启动子的质粒中，并在大肠杆菌中高效表达。
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