Muel A S, Chaudun E, Courtois Y, Modak S P, Counis M F
J Cell Physiol. 1986 Apr;127(1):167-74. doi: 10.1002/jcp.1041270120.
During terminal differentiation of lens epithelial cells into fiber cells, nuclei become pycnotic and DNA degradation occurs. We investigated the putative role in this process of an endogenous DNAase. After incubation of isolated nuclei of both cell types at 37 degrees C, DNAase activity was revealed by DNA size analysis on 0.3-1% neutral and alkaline agarose, one- and two-dimensional gels. This DNAase activity is more prominent in lens fiber nuclei than in epithelial nuclei at all the embryonic stages probably because of a preexisting higher concentration of divalent cations in the former. This activity is calcium or magnesium dependent in both types of nuclei.
在晶状体上皮细胞向纤维细胞的终末分化过程中，细胞核会固缩，同时发生DNA降解。我们研究了内源性DNA酶在这一过程中的假定作用。将两种细胞类型的分离细胞核在37℃孵育后，通过在0.3 - 1%的中性和碱性琼脂糖、一维及二维凝胶上进行DNA大小分析来揭示DNA酶活性。在所有胚胎阶段，这种DNA酶活性在晶状体纤维细胞核中比在上皮细胞核中更为显著，这可能是因为前者中预先存在较高浓度的二价阳离子。两种细胞核中的这种活性均依赖于钙或镁。
