Transductional analysis of the cysII region of Pseudomonas aeruginosa.

The close linkage between hisI and mutations of the cysII group in Pseudomonas aeruginosa was confirmed by mapping experiments with new mutants. All mutants of the cysII group were blocked in the cysteine biosynthesis before sulfite (this include the mutant cys-5605 which had previously been mapped by Mee and Lee, 1969). Most of the sulfite requiring cys mutants belonged to this group. The farthest distance between two mutations of the cysII group was calculated by the Wu formula to be approximately 2.5 kbp. We, therefore, suggest that more than one gene of the cysteine pathway may be located within this region.