Matsumoto T, Himeno K, Mitani M, Mori K, Miake S, Nomoto K
J Clin Lab Immunol. 1986 Feb;19(2):83-9.
The growth of Meth A (MA) tumors was suppressed in tumor-antigen-specific manner in BALB/c mice immunized with mitomycin C-treated MA (MMC-MA) cells in saline or in Freund's complete adjuvant (FCA). The antitumor activity of their peritoneal exudate cells (PEC) was detected by in vivo neutralization test and in vitro cytostasis assay but not by in vitro cytolysis assay. Positive delayed footpad reaction was elicited by footpad injection of MMC-MA cells in such immunized mice, before or after the inoculation of viable MA cells. Expression of cytostatic activity in PEC required the interaction of non-adherent and adherent cells. Normal PEC could exert the cytostatic activity in the presence of a nonadherent population of immune PEC. These findings suggest that cytostatic macrophages are activated after the interaction between sensitized lymphocytes and tumor-specific antigens and that they play an important role in the principal effector mechanism in this syngeneic system. On the other hand, immunization with MMC-MA in FCA or viable MA cells also induced PEC the antitumor activity detected by in vivo neutralization test in allogeneic C57BL/6 hosts. Immunization with viable MA cells induced not only cytolytic but also cytostatic activity, whereas, immunization with MMC-MA in FCA induced cytostatic activity but not cytolytic activity. In contrast to a syngeneic system, cytolytic activity was effectively induced against allogeneic MA cells together with cytostatic activity. We conclude that there are various effector cells contributing to the elimination of syngeneic or allogeneic murine tumor cells.
在以生理盐水或弗氏完全佐剂(FCA)中经丝裂霉素C处理的甲基胆蒽(MA)细胞免疫的BALB/c小鼠中,MA肿瘤的生长以肿瘤抗原特异性方式受到抑制。通过体内中和试验和体外细胞生长抑制试验检测了其腹腔渗出细胞(PEC)的抗肿瘤活性,但未通过体外细胞溶解试验检测。在接种活的MA细胞之前或之后,通过足垫注射MMC-MA细胞在这种免疫小鼠中引发了阳性迟发型足垫反应。PEC中细胞生长抑制活性的表达需要非贴壁细胞和贴壁细胞的相互作用。正常PEC在存在免疫PEC的非贴壁群体时可发挥细胞生长抑制活性。这些发现表明,细胞生长抑制性巨噬细胞在致敏淋巴细胞与肿瘤特异性抗原相互作用后被激活,并且它们在该同基因系统的主要效应机制中起重要作用。另一方面,用FCA中的MMC-MA或活的MA细胞免疫也在同种异体C57BL/6宿主中诱导了通过体内中和试验检测到的PEC的抗肿瘤活性。用活的MA细胞免疫不仅诱导了细胞溶解活性,还诱导了细胞生长抑制活性,而用FCA中的MMC-MA免疫诱导了细胞生长抑制活性但未诱导细胞溶解活性。与同基因系统相反,针对同种异体MA细胞有效地诱导了细胞溶解活性以及细胞生长抑制活性。我们得出结论,有多种效应细胞有助于消除同基因或同种异体小鼠肿瘤细胞。