Zebrafish behavioral phenomics applied for phenotyping aquatic neurotoxicity induced by lead contaminants of environmentally relevant level.

Environmental lead (Pb) exposure is a worldwide threat due to the ubiquitous contamination. Although the adverse effects of Pb on human health have previously been extensively explored, the eco-toxicological effects on aquatic vertebrates still need further investigation. In addition, there is a paucity in the knowledge of behavioral and physiological effects of Pb within the range of environmental relevant concentration (under 100 μg/L) on aquatic organisms such as zebrafish. Herein, we demonstrated that adult male zebrafish (Danio rerio) exposed to Pb at environmental concentration level (1 μg/L, 10 μg/L and 100 μg/L) for 14 days, exhibited obvious neuro-behavioral alteration including disturbed light dark preference, impaired exploratory behaviors and inhibited spatial working memory. The alteration of entire behavioral profiles was further associated with the disturbed expression patterns of mRNA level of key genes involved in neurodevelopment (gap43, syn2a, th, dat, and drd1b), neurotoxic effects (c-fos and gfap), and stress responses (tap, mt1, hsp70, and hsp90). To determine the comprehensively effect of aquatic contaminants on the entire behavioral profiles, behavioral phenomic data were obtained by hierarchical clustering analysis. Overall, we employed behavioral phenomics methods to find that Pb within standard chronic Pb toxic criteria, significantly altered behavioral phenotype and brain physiology, which would exert profound ecological consequences and offer the reference for adjustment of aquatic toxic criteria.