一种用于野油菜黄单胞菌莴苣致病变种（生菜细菌性叶斑病的病原菌）的新型半选择性培养基。

A New Semi-Selective Medium for Xanthomonas campestris pv. vitians, the Causal Agent of Bacterial Leaf Spot of Lettuce.

作者信息

Toussaint V, Morris C E, Carisse O

机构信息

MacDonald Campus of McGill University, 21 111 Lakeshore, Ste-Anne-de-Bellevue, Quebec, H9X 3V9, Canada.

Station de Pathologie Végétale, INRA d' Avignon, 84143, Monfavet, Cedex, France.

出版信息

Plant Dis. 2001 Feb;85(2):131-136. doi: 10.1094/PDIS.2001.85.2.131.

DOI:10.1094/PDIS.2001.85.2.131

PMID:30831931

Abstract

A semi-selective medium containing maltose, methyl green, and antibiotics (MMG) was developed for the isolation of Xanthomonas campestris pv. vitians. The semi-selective medium was evaluated based on plating efficiency of X. campestris pv. vitians in cell suspensions of pure cultures from leaves and soil. MMG medium allowed recovery of 5.7 to 30.6% of the X. campestris pv. vitians colonies recovered on nutrient agar and 0.1 to 8.4% of those recovered on 1/10-strength tryptic soy agar. MMG inhibited growth of most background bacteria and allowed reliable identification of X. campestris pv. vitians. The semi-selective medium contained (per liter) maltose (10 g), tryptone (5 g), KHPO (3.5 g), KHPO (2.75 g), trace elements (0.02 to 1.0 mg), methyl green (2 ml of a 1% aqueous solution), amoxicillin (32 mg), cephalothin (32 mg), cycloheximide (50 mg), and agar (15 g).

摘要

一种含有麦芽糖、甲基绿和抗生素的半选择性培养基（MMG）被开发用于分离野油菜黄单胞菌黄瓜致病变种。基于野油菜黄单胞菌黄瓜致病变种在来自叶片和土壤的纯培养物细胞悬液中的平板接种效率，对该半选择性培养基进行了评估。MMG培养基能回收在营养琼脂上回收的野油菜黄单胞菌黄瓜致病变种菌落的5.7%至30.6%，以及在1/10强度胰蛋白胨大豆琼脂上回收的菌落的0.1%至8.4%。MMG抑制了大多数背景细菌的生长，并能可靠地鉴定野油菜黄单胞菌黄瓜致病变种。该半选择性培养基每升含有麦芽糖（10克）、胰蛋白胨（5克）、KHPO（3.5克）、KHPO（2.75克）、微量元素（0.02至1.0毫克）、甲基绿（2毫升1%水溶液）、阿莫西林（32毫克）、头孢噻吩（32毫克）、环己酰亚胺（50毫克）和琼脂（15克）。