George Tijith K, Houbraken Jos, Mathew Linu, Jisha M S
School of Biosciences, Mahatma Gandhi University, Kottayam, India.
Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands.
Mycology. 2018 Dec 12;10(1):49-60. doi: 10.1080/21501203.2018.1555868. eCollection 2019 Mar.
Medicinal plants are considered as sources of novel and unexplored groups of endophytic microorganisms. A study on endophytic fungal species from the medicinal plant (L.) Dunal resulted in the isolation of a isolate (WSR 62) with antibiotic activity. Phylogenetic analysis showed that the isolate belongs to section , and it is most closely related to . Subsequent detailed phylogenetic analyses using partial β-tubulin (), calmodulin () and DNA-dependent RNA polymerase II () gene sequences of a larger number of related strains revealed the distinctiveness of the isolate in the clade. The isolate grows fast on Czapek yeast autolysate agar (CYA) and malt extract agar (MEA) incubated at 25°C, 30°C and 37°C. The obverse colony colour is dominated by the conspicuous production of cleistothecia and is greyish yellow on CYA and yellowish brown on MEA. Production of cleistothecia containing prominent spinose ascospores was present on all tested agar media. Based on the phylogenetic analysis and the phenotypic characterisation, strain WSR 62 from is described here as a novel species named
药用植物被认为是新型且未被探索的内生微生物群体的来源。一项对药用植物(L.)杜纳尔内生真菌物种的研究导致分离出一种具有抗生素活性的分离株(WSR 62)。系统发育分析表明,该分离株属于某组,并且与……关系最为密切。随后,使用大量相关菌株的部分β-微管蛋白()、钙调蛋白()和依赖DNA的RNA聚合酶II()基因序列进行的详细系统发育分析揭示了该分离株在进化枝中的独特性。该分离株在25°C、30°C和37°C下培养的查氏酵母自溶物琼脂(CYA)和麦芽提取物琼脂(MEA)上生长迅速。正面菌落颜色以明显产生闭囊壳为主,在CYA上为灰黄色,在MEA上为黄棕色。在所有测试的琼脂培养基上均存在含有突出具刺子囊孢子的闭囊壳。基于系统发育分析和表型特征,这里将来自……的菌株WSR 62描述为一个名为……的新物种