Nigg E A, Sefton B M, Singer S J, Vogt P K
Virology. 1986 May;151(1):50-65. doi: 10.1016/0042-6822(86)90103-0.
Neoplastic transformation of fibroblasts results in widely different cell morphologies. We have attempted to correlate cell morphology with cytoskeletal organization and fibronectin expression in murine and avian fibroblasts transformed by a diverse group of viral and chemical agents. The distribution of vinculin, alpha-actinin, actin, and surface fibronectin was studied, and, where appropriate, also the extent of phosphotyrosine modification of vinculin. Irrespective of the transforming agent we found that increased cell rounding was generally correlated with a reduction in vinculin-containing focal adhesions, a dissolution of microfilament bundles, and a reduction of extracellular fibronectin. In contrast, spindle-shaped fibroblasts expressed relatively high levels of surface fibronectin. Reorganization of vinculin, actin, and alpha-actinin into rosette-like structures was observed in polygonal or rounded cells transformed by viruses encoding tyrosine kinases, but was not seen in fibroblasts transformed by agents without associated tyrosine kinase activity or in spindle-shaped cells. No correlation was found between the extent of phosphotyrosine modification of vinculin and the extent of cell rounding. Irrespective of cell morphology, the extent of tyrosine phosphorylation of vinculin was high in all cells transformed by viruses carrying the src gene, but low in those transformed by viruses expressing the fps gene. Our results indicate that the morphology of a transformed cell is determined by a combination of several factors which are affected to different extents by different transforming agents.
成纤维细胞的肿瘤转化会导致广泛不同的细胞形态。我们试图将细胞形态与细胞骨架组织以及在被多种病毒和化学试剂转化的小鼠和禽类成纤维细胞中的纤连蛋白表达相关联。研究了纽蛋白、α-辅肌动蛋白、肌动蛋白和表面纤连蛋白的分布，并且在适当情况下还研究了纽蛋白的磷酸酪氨酸修饰程度。无论转化因子如何，我们发现细胞变圆程度的增加通常与含纽蛋白的粘着斑减少、微丝束的溶解以及细胞外纤连蛋白的减少相关。相反，纺锤形成纤维细胞表达相对高水平的表面纤连蛋白。在由编码酪氨酸激酶的病毒转化的多边形或圆形细胞中观察到纽蛋白、肌动蛋白和α-辅肌动蛋白重组成玫瑰花结样结构，但在由无相关酪氨酸激酶活性的试剂转化的成纤维细胞或纺锤形细胞中未观察到。未发现纽蛋白的磷酸酪氨酸修饰程度与细胞变圆程度之间存在相关性。无论细胞形态如何，在所有由携带src基因的病毒转化的细胞中，纽蛋白的酪氨酸磷酸化程度都很高，但在由表达fps基因的病毒转化的细胞中则很低。我们的结果表明，转化细胞的形态由多种因素共同决定，不同的转化因子对这些因素的影响程度不同。
