Dahl C A, Matthews M E, Marth E H
Department of Food Science, University of Wisconsin-Madison, Madison, Wisconsin 53706.
J Food Prot. 1981 Feb;44(2):128-133. doi: 10.4315/0362-028X-44.2.128.
This study evaluated survival of a radiation-resistant organism, Streptococcus faecium , as a potential indication of safety of beef loaf and potatoes after microwave-heating in a hospital cook/chill foodservice system. The cook/chill system was simulated in a laboratory three times for both beef loaf and potatoes. Beef loaf (15 kg) was initially cooked to 66 C in a convection oven (156 C), chilled 24 h at 6 C, cut into 100-g portions, inoculated on the external surface with S. faecium (1 × 10 CFU/g), chilled 2 h, and microwave-heated for 20, 50, 80 or 110 sec. Dehydrated potatoes (5.7 kg) were reconstituted to 79 C over direct heat, chilled to 37 C, inoculated internally with S. faecium (1 × 10 CFU/g), stored chilled 24 h at 6 C, portioned into 100 g/portion, chilled 2 h at 6 C and microwave-heated for 25, 45, 65 or 85 sec. Results indicated that increasing the time of microwave-heating for portions of beef loaf or potatoes decreased the count on KF agar regardless of internal or external inoculation procedure. Eighty or 85 sec of microwave-heating of beef loaf or potatoes resulted in portions with similar numbers of bacteria as determined by KF agar, but mean internal end temperatures ranged from 66 to 95 C.
本研究评估了耐辐射微生物粪肠球菌的存活情况,以此作为医院烹饪/冷藏食品服务系统中微波加热后牛肉糕和土豆安全性的潜在指标。牛肉糕和土豆的烹饪/冷藏系统在实验室中模拟了三次。牛肉糕(15千克)最初在对流烤箱(156℃)中煮至66℃,在6℃下冷藏24小时,切成100克的份块,在外表面接种粪肠球菌(1×10CFU/克),冷藏2小时,然后微波加热20、50、80或110秒。脱水土豆(5.7千克)直接加热复水至79℃,冷却至37℃,在内部接种粪肠球菌(1×10CFU/克),在6℃下冷藏储存24小时,分成100克/份,在6℃下冷藏2小时,然后微波加热25、45、65或85秒。结果表明,无论采用内部还是外部接种程序,增加牛肉糕或土豆块的微波加热时间都会降低KF琼脂上的菌数。牛肉糕或土豆微波加热80或85秒后,KF琼脂检测的细菌数量相似,但内部最终平均温度范围为66至95℃。