Liotti F S, Bodo M, Pezzetti F, Guerrieri P, Menghini A R
Oncology. 1986;43(3):183-6. doi: 10.1159/000226360.
A study on the capacity of ascorbic acid, reduced glutathione and cysteine to interfere with 3H-7,12-dimethylbenz[a]anthracene (3H-DMBA) binding to DNA in cultured fibroblast-like cells from 11-day-old chick embryos showed that, although the total amount of 3H-DMBA in the treated cells was greater than in the untreated cells, the DNA-bound 3H-DMBA was less. Comparisons between the various experimental groups demonstrated that the greater 3H-DMBA in the ascorbic acid-, reduced glutathione-, and cysteine-treated groups could not be attributed to an initially higher number of cells, nor to a treatment-induced increase in DNA synthesis. It is proposed that the three substances examined inhibit the oxidative degradation of 3H-DMBA, thereby favoring its accumulation within the cell and reducing the formation of DNA-binding metabolites.
一项关于抗坏血酸、还原型谷胱甘肽和半胱氨酸干扰3H-7,12-二甲基苯并[a]蒽（3H-DMBA）与11日龄鸡胚培养的成纤维样细胞中DNA结合能力的研究表明，尽管处理过的细胞中3H-DMBA的总量高于未处理的细胞，但与DNA结合的3H-DMBA较少。各实验组之间的比较表明，抗坏血酸、还原型谷胱甘肽和半胱氨酸处理组中较高的3H-DMBA量既不能归因于最初细胞数量较多，也不能归因于处理诱导的DNA合成增加。有人提出，所研究的这三种物质会抑制3H-DMBA的氧化降解，从而有利于其在细胞内积累并减少DNA结合代谢物的形成。
