Plant Imaging and Mass Spectrometry (PIMS), Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France.
Plant Imaging and Mass Spectrometry (PIMS), Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France; Département mécanique, ICube Laboratoire des sciences de l'ingénieur, de l'informatique et de l'imagerie, UNISTRA/CNRS/ENGEES/INSA, 2 rue Boussingault, 67000 Strasbourg, France.
Environ Int. 2019 May;126:523-532. doi: 10.1016/j.envint.2019.02.066. Epub 2019 Mar 6.
Micropollutants and emerging organic contaminants (EOCs) have been widely studied in terms of persistance, removal, human risk assessment, toxicology, etc. Mass spectrometry imaging (MSI) offers the possibility of following the fate of a single pesticide in a plant leaf or a drug in the whole body of an animal, organ by organ. However, the admissibility of chronic low doses of complex mixtures for the ecosystem has not been assessed. How do micropollutants diffuse in the environment? How do living organisms cope with chronic exposure to a low dose of diverse micropollutants? Is there a cocktail effect or a chance for hormesis? Combining mass spectrometry imaging (MSI) and targeted and nontargeted liquid chromatography coupled to mass spectrometry (LC-MS), we attempt to answer these questions. We investigate the diversity of micropollutants at the exit of a water treatment facility, their diffusion in sludge and black poplar (Populus nigra), and their impact on a living organism. We reveal a specific tissue localization of micropollutants in peripheral leaf tissues, and an associated stress response from the plant, with stress hormones and tissue degradation markers induced in the plant growing near the water efflux.
微污染物和新兴有机污染物（EOCs）在持久性、去除、人类风险评估、毒理学等方面得到了广泛的研究。质谱成像（MSI）提供了在植物叶片中跟踪单个农药或动物体内药物在各个器官中命运的可能性。然而，对于生态系统来说，复杂混合物的慢性低剂量的可接受性尚未得到评估。微污染物如何在环境中扩散？生物体如何应对慢性暴露于低剂量多种微污染物？是否存在鸡尾酒效应或适应低剂量效应？我们结合质谱成像（MSI）和靶向及非靶向液相色谱与质谱联用（LC-MS），尝试回答这些问题。我们研究了水处理设施出口处的微污染物的多样性，它们在污泥和黑杨（Populus nigra）中的扩散，以及它们对生物体的影响。我们揭示了微污染物在叶片外周组织中的特定组织定位，以及植物的应激反应，在靠近水出口处生长的植物中诱导了应激激素和组织降解标志物。
