Tian Wei, Qiu Zheng, Cui Yuchao, Zhang Jing, Ma Xingjie, Cui Shangjin, Zheng Shimin
Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, College of Veterinary Medicine, Northeast Agricultural University, No.59 Mucai street, Harbin, People's Republic of China / State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Haping Street, Harbin, People's Republic of China.
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Haping Street, Harbin, People's Republic of China.
Pak J Pharm Sci. 2019 Jan;32(1(Special)):377-382.
Laboratory-prepared inactivated porcine parvovirus (PPV) vaccines and VP2 virus-like particles (VLPs) were utilized to immunize gilts. PPV BQ strain and SP2/0 cells were used. Hemagglutination-inhibiting (HI) antibody titers were measured in the immunized gilts and the differences in cytokine production of interferon gamma (IFN-γ, IL-2 and IL-4) were compared. CD4 and CD8 T cells proliferation were compared by flow cytometry. The variation between the immune response level induced by inactivated PPV vaccine and VP2 VLPs were determined. The results showed that all vaccinated gilts had HI antibody titers reaching 1:256 for at least one month post immunization and the peak level of antibody could be sustained for one month; further, PPV antibodies could be detected in the second week post immunization with VP2 VLPs. We also found that the level of cytokines (IFN-γ, IL-2 and IL-4) were all increased post immunization and continued to rise after the booster immunization; the level of increase in IFN-γ and IL-2 were significantly higher than IL-4. The flow cytometry results showed that the numbers of the CD4+ and CD8+ T cells subsets were significantly higher in the groups immunized with inactivated PPV vaccine or VP2 VLPs than those of negative control group (p<0.01); additionally, the number of CD4 cells in the gilts that received VP2 VLP immunization was significantly higher than the inactivated vaccine group (p<0.01). In summary, the inactivated PPV vaccine and PPV VP2 VLPs were both able to induce humoral and cellular immunity, but the VP2 VLPs lead to better cellular immune responses in gilts compared to those of the inactivated vaccine..
利用实验室制备的灭活猪细小病毒（PPV）疫苗和VP2病毒样颗粒（VLP）对后备母猪进行免疫。使用PPV BQ株和SP2/0细胞。测定免疫后备母猪的血凝抑制（HI）抗体效价，并比较干扰素γ（IFN-γ、IL-2和IL-4）细胞因子产生的差异。通过流式细胞术比较CD4和CD8 T细胞增殖情况。确定灭活PPV疫苗和VP2 VLP诱导的免疫反应水平之间的差异。结果表明，所有接种疫苗的后备母猪在免疫后至少1个月内HI抗体效价达到1:256，且抗体峰值水平可持续1个月；此外，用VP2 VLP免疫后第2周可检测到PPV抗体。我们还发现，免疫后细胞因子（IFN-γ、IL-2和IL-4）水平均升高，加强免疫后继续上升；IFN-γ和IL-2的升高水平显著高于IL-4。流式细胞术结果显示，用灭活PPV疫苗或VP2 VLP免疫的组中CD4+和CD8+ T细胞亚群数量显著高于阴性对照组（p<0.01）；此外，接受VP2 VLP免疫的后备母猪中CD4细胞数量显著高于灭活疫苗组（p<0.01）。综上所述，灭活PPV疫苗和PPV VP2 VLP均能诱导体液免疫和细胞免疫，但与灭活疫苗相比，VP2 VLP在后备母猪中诱导的细胞免疫反应更好。
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