[一种化学斑疹伤寒疫苗及从普氏立克次体获得的颗粒放射性抗原的免疫原性]

[Immunogenicity of a chemical typhus vaccine and of a corpuscular radioantigen obtained from Rickettsia prowazekii].

作者信息

Pushkareva V I, Kekcheeva N G, Kokorin I N

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1986 Jan(1):73-6.

Abstract

The protective activity of chemical typhus vaccine and R. prowazekii corpuscular radioantigen (CRA) was studied. Guinea pigs were immunized with doses of 32 and 48 antigenic units. Antibody production was assayed in the complement fixation test. On days 7, 15, 21, 30 and 60 after immunization the animals were challenged with R. prowazekii introduced in an amount of 10(5) minimum embryonal infective doses (MEID). On day 30 some of the animals were challenged with 10(3) MEID of R. typhi. The results demonstrated that both preparations were highly immunogenic and capable of protecting most of the animals from 10(5) MEID of R. prowazekii. Immunity developed earlier after immunization with CRA. The guinea pigs immunized with CRA, purified in percoll density gradient, and challenged with 10(3) MEID of R. typhi on day 30 showed a high level of cross immunity. In all control animals high fever and periorchitis were observed.

摘要

研究了化学性斑疹伤寒疫苗和普氏立克次体颗粒放射性抗原（CRA）的保护活性。用32和48个抗原单位的剂量对豚鼠进行免疫。在补体结合试验中测定抗体产生情况。免疫后第7、15、21、30和60天，用10⁵最小胚胎感染剂量（MEID）的普氏立克次体对动物进行攻击。在第30天，部分动物用10³MEID的伤寒立克次体进行攻击。结果表明，两种制剂均具有高度免疫原性，能够保护大多数动物免受10⁵MEID普氏立克次体的感染。用CRA免疫后免疫反应出现得更早。用在Percoll密度梯度中纯化的CRA免疫并在第30天用10³MEID伤寒立克次体攻击的豚鼠表现出高水平的交叉免疫。在所有对照动物中均观察到高热和睾丸炎。