Huang Y H, Hartman G L
Department of Crop Sciences, University of Illinois at Urbana-Champaign (UIUC).
USDA, Agricultural Research Service, and Department of Crop Sciences, UIUC, Urbana, IL 61801-4723.
Plant Dis. 1998 Sep;82(9):999-1002. doi: 10.1094/PDIS.1998.82.9.999.
Four soybean plant introductions, PI 520.733, PI 567.374, PI 567.650B, and PI 567.659, and one soybean cultivar, Great Lakes 3202, were inoculated under greenhouse conditions with four isolates of Fusarium solani f. sp. glycines. Foliar disease severity rating was greatest on PI 567.659, followed by Great Lakes 3202, PI 520.733, PI 567.650B, and PI 567.374. There was no significant interaction between isolates and soybean entries for foliar disease severity ratings. Experiments also were conducted to determine if disease development and root colonization differed among entries. Root infection of the five entries did not differ (P = 0.05). Foliar disease progress curves increased faster for PI 567.659 and Great Lakes 3202 than for PI 567.374. The area under the disease progress curve (AUDPC) value for PI 567.374 was the lowest and differed (P = 0.01) from AUDPC values for Great Lakes 3202 and PI 567.659. There were no differences (P = 0.01) in length of taproot lesions, losses in root dry weight, and vascular stem length discoloration among the entries, and there was no correlation (P = 0.05) between these measurements and foliar AUDPC values. Cut seedling stems immersed in culture filtrate developed interveinal chlorosis on leaves of each entry within 2 days. Disease severity on cut seedlings of PI 567.374 was lower (P = 0.01) than on the other entries. There was a positive correlation (r = 0.94, P = 0.05) between AUDPC values of the five entries inoculated with the fungus and the cut seedling test using culture filtrate.
对四个大豆引进品种PI 520.733、PI 567.374、PI 567.650B和PI 567.659以及一个大豆品种大湖3202在温室条件下接种四种大豆尖孢镰刀菌分离株。PI 567.659的叶部病害严重程度评级最高，其次是大湖3202、PI 520.733、PI 567.650B和PI 567.374。分离株与大豆材料之间在叶部病害严重程度评级上没有显著交互作用。还进行了试验以确定不同材料间病害发展和根部定殖情况是否存在差异。五个材料的根部感染情况没有差异（P = 0.05）。PI 567.659和大湖3202的叶部病害进展曲线比PI 567.374上升得更快。PI 567.374的病害进展曲线下面积（AUDPC）值最低，且与大湖3202和PI 567.659的AUDPC值不同（P = 0.01）。各材料在主根病斑长度、根干重损失和茎维管束长度变色方面没有差异（P = 0.01），并且这些测量值与叶部AUDPC值之间没有相关性（P = 0.05）。将幼苗茎段浸入培养滤液中，2天内每个材料的叶片都出现了脉间失绿。PI 567.374的切段幼苗病害严重程度低于其他材料（P = 0.01）。接种真菌的五个材料的AUDPC值与使用培养滤液的切段幼苗试验之间存在正相关（r = 0.94，P = 0.05）。
