Schulman M D, Valentino D, Hensens O
J Antibiot (Tokyo). 1986 Apr;39(4):541-9. doi: 10.7164/antibiotics.39.541.
The biosynthesis of the avermectins, a group of 16 membered macrolides with potent anthelmintic and insecticidal activity produced by Streptomyces avermitilis, was studied by supplying cultures with 14C and 13C precursors. [1-14C] and [2-14C]acetate and propionate were poor precursors of the avermectins and were instead rapidly oxidized to 14CO2. The S-methyl of methionine in contrast was incorporated extensively and equally into the three methoxyl groups of the avermectins. The carbon backbone of methionine was not a precursor of the avermectins. Feeding of [1-13C]glucose yielded avermectins labeled specifically in the C1' and C1" of the oleandrose moiety and in the aglycone moiety in carbons known to be derived from the methyl of acetate. Feeding [U-13C]glucose showed that the entire avermectin molecule is derived from glucose carbons.
通过向阿维链霉菌培养物提供¹⁴C和¹³C前体，研究了阿维菌素（由阿维链霉菌产生的一组具有强大驱虫和杀虫活性的16元大环内酯类化合物）的生物合成。[1-¹⁴C]和[2-¹⁴C]乙酸盐和丙酸盐是阿维菌素的不良前体，反而会迅速氧化为¹⁴CO₂。相比之下，甲硫氨酸的S-甲基被广泛且等量地掺入到阿维菌素的三个甲氧基中。甲硫氨酸的碳骨架不是阿维菌素的前体。用[1-¹³C]葡萄糖喂养产生了在夹竹桃糖部分的C1'和C1"以及已知来自乙酸盐甲基的碳的苷元部分中特异性标记的阿维菌素。用[U-¹³C]葡萄糖喂养表明整个阿维菌素分子都来自葡萄糖碳。
