Napolitani Giorgio, Kurupati Prathiba, Teng Karen Wei Weng, Gibani Malick M, Rei Margarida, Aulicino Anna, Preciado-Llanes Lorena, Wong Michael Thomas, Becht Etienne, Howson Lauren, de Haas Paola, Salio Mariolina, Blohmke Christoph J, Olsen Lars Rønn, Pinto David Miguel Susano, Scifo Laura, Jones Claire, Dobinson Hazel, Campbell Danielle, Juel Helene B, Thomaides-Brears Helena, Pickard Derek, Bumann Dirk, Baker Stephen, Dougan Gordon, Simmons Alison, Gordon Melita A, Newell Evan William, Pollard Andrew J, Cerundolo Vincenzo
MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
Singapore Immunology Network, Agency of Science, Technology and Research, Singapore, Singapore.
Nat Immunol. 2019 Apr;20(4):514. doi: 10.1038/s41590-019-0357-6.
In the version of this article initially published, the first affiliation lacked 'MRC'; the correct name of the institution is 'MRC Weatherall Institute of Molecular Medicine'. Two designations (SP110Y and ST110H) were incorrect in the legend to Fig. 6f,h,i. The correct text is as follows: for panel f, "...loaded with either the CdtB(105-125)SP110Y (DRB4SP110Y) or the CdtB(105-125)ST110H (DRB4ST110H) peptide variants..."; for panel h, "...decorated by the DRB4SP110Y tetramer (lower-right quadrant), the DRB4ST110H (upper-left quadrant)..."; and for panel i, "...stained ex vivo with DRB4SP110Y, DRB4ST110H...". In Fig. 8e, the final six residues (LTEAFF) of the sequence in the far right column of the third row of the table were missing; the correct sequence is 'CASSYRRTPPLTEAFF'. In the legend to Fig. 8d, a designation (HLyE) was incorrect; the correct text is as follows: "(HlyE?)." Portions of the Acknowledgements section were incorrect; the correct text is as follows: "This work was supported by the UK Medical Research Council (MRC) (MR/K021222/1) (G.N., M.A.G., A.S., V.C., A.J.P.),...the Oxford Biomedical Research Centre (A.J.P., V.C.),...and core funding from the Singapore Immunology Network (SIgN) (E.W.N.) and the SIgN immunomonitoring platform (E.W.N.)." Finally, a parenthetical element was phrased incorrectly in the final paragraph of the Methods subsection "T cell cloning and live fluorescence barcoding"; the correct phrasing is as follows: "...(which in all cases included HlyE, CdtB, Ty21a, Quailes, NVGH308, and LT2 strains and in volunteers T5 and T6 included PhoN)...". Also, in Figs. 3c and 4a, the right outlines of the plots were not visible; in the legend to Fig. 3, panel letter 'f' was not bold; and in Fig. 8f, 'ND' should be aligned directly beneath DRB4 in the key and 'ND' should be removed from the diagram at right, and the legend should be revised accordingly as follows: "...colors indicate the HLA class II restriction (gray indicates clones for which restriction was not determined (ND)). Clonotypes are grouped on the basis of pathogen selectivity (continuous line), protein specificity (dashed line) and epitope specificity; for ten HlyE-specific clones (pixilated squares), the epitope specificity was not determined...". The errors have been corrected in the HTML and PDF versions of the article.
在本文最初发表的版本中,第一个单位名称缺少“MRC”;该机构的正确名称是“医学研究理事会韦瑟罗尔分子医学研究所(MRC Weatherall Institute of Molecular Medicine)”。图6f、h、i的图例中有两个标注(SP110Y和ST110H)错误。正确的文本如下:对于图f面板,“……负载有CdtB(105 - 125)SP110Y(DRB4SP110Y)或CdtB(105 - 125)ST110H(DRB4ST110H)肽变体……”;对于图h面板,“……由DRB4SP110Y四聚体(右下角象限)、DRB4ST110H(左上角象限)标记……”;对于图i面板,“……用DRB4SP110Y、DRB4ST110H进行离体染色……”。在图8e中,表格第三行最右列序列的最后六个残基(LTEAFF)缺失;正确的序列是“CASSYRRTPPLTEAFF”。在图8d的图例中,一个标注(HLyE)错误;正确的文本如下:“(HlyE?)”。致谢部分的一些内容有误;正确的文本如下:“本研究得到了英国医学研究理事会(MRC)(MR/K021222/1)(G.N.、M.A.G.、A.S.、V.C.、A.J.P.)、……牛津生物医学研究中心(A.J.P.、V.C.)、……以及新加坡免疫网络(SIgN)(E.W.N.)的核心资金支持和SIgN免疫监测平台(E.W.N.)。”最后,在“T细胞克隆与活荧光条形码标记”方法小节的最后一段中,一个插入语的表述有误;正确的表述如下:“……(在所有情况下包括HlyE、CdtB、Ty21a、Quailes、NVGH308和LT2菌株,在志愿者T5和T6中还包括PhoN)……”。此外,在图3c和4a中,图的右轮廓不可见;在图3的图例中,面板字母“f”未加粗;在图8f中,“ND”应在图例键中直接对齐DRB4下方,并且应从右侧图中移除“ND”,图例应相应修改如下:“……颜色表示HLA II类限制性(灰色表示未确定限制性的克隆(ND))。克隆型根据病原体选择性(实线)、蛋白质特异性(虚线)和表位特异性进行分组;对于十个HlyE特异性克隆(像素化方块),表位特异性未确定……”。这些错误已在文章的HTML和PDF版本中得到纠正。