Kasama T, Seyama Y
J Biochem. 1986 Mar;99(3):771-5. doi: 10.1093/oxfordjournals.jbchem.a135536.
We developed a simple quantitative procedure for cholestanol in serum involving reversed phase thin layer chromatography. This procedure was satisfactory with regard to the linearity of the calibration curve in the range of 100 ng to 1,000 ng, recovery and reproducibility. Only 100 microliter of serum was needed for determination of the cholestanol concentration. Prior to thin layer chromatography, cholesterol was converted to alpha- and beta-epoxides with m-chloroperbenzoic acid, which were clearly distinguishable from cholestanol on TLC. Detection of sterols was performed by spraying with phosphomolybdic acid solution. Quantification of cholestanol was carried out with a TLC scanning densitometer. When serum cholestanol in cerebrotendinous xanthomatosis (CTX) patients was quantified by TLC, GC-MS, and GC, the correlation among the three methods was found to be approximately 1:1:1. It was found that the present method was useful for the primary diagnostic screening of CTX because of its simplicity and because many samples could be analyzed at one time.
我们开发了一种用于血清中胆甾烷醇的简单定量方法，该方法涉及反相薄层色谱法。该方法在校准曲线100纳克至1000纳克范围内的线性、回收率和重现性方面令人满意。测定胆甾烷醇浓度仅需100微升血清。在薄层色谱分析之前，用间氯过苯甲酸将胆固醇转化为α-和β-环氧化物，它们在薄层色谱上与胆甾烷醇明显可区分。通过喷洒磷钼酸溶液进行甾醇检测。用薄层色谱扫描密度计对胆甾烷醇进行定量。当通过薄层色谱法、气相色谱-质谱联用仪和气相色谱对脑腱性黄瘤病（CTX）患者的血清胆甾烷醇进行定量时，发现这三种方法之间的相关性约为1:1:1。发现本方法因其简单性以及能够一次性分析多个样品，对CTX的初步诊断筛查很有用。
