Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, 401331, Chongqing, China.
Department of Plant Sciences, University of California, Davis, CA, 95616, USA.
Sci Rep. 2019 Mar 14;9(1):4470. doi: 10.1038/s41598-019-41065-9.
Tomato fruit are especially susceptible to chilling injury (CI) when continuously exposed to temperatures below 12 °C. In this study, integrative comparative analyses of transcriptomics and metabolomics data were performed to uncover the regulatory network in CI tomato fruit. Metabolite profiling analysis found that 7 amino acids, 27 organic acids, 16 of sugars and 22 other compounds had a significantly different content while transcriptomics data showed 1735 differentially expressed genes (DEGs) were down-regulated and 1369 were up-regulated in cold-stored fruit. We found that the contents of citrate, cis-aconitate and succinate were increased, which were consistent with the expression of ATP-citrate synthase (ACS) and isocitrate dehydrogenase (IDH) genes in cold-treated tomato fruit. Cold stress promotes the expression of ACS and IDH which may increase the synthesis of citrate, cis-aconitate and succinate. Alanine and leucine had increased contents, which may result from alanine aminotransferase (ALT) and branched-chain amino acid aminotransferase (BcAT)'s high expression levels, respectively. Overall the transcriptomics and metabolomics data in our study explain the molecular mechanisms of the chilling injury and expands our understanding of the complex regulatory mechanisms of a metabolic network in response to chilling injury in tomato fruit.
番茄果实连续暴露在 12°C 以下的温度下时,特别容易受到冷害(CI)的影响。在这项研究中,我们对转录组学和代谢组学数据进行了综合比较分析,以揭示 CI 番茄果实中的调控网络。代谢物谱分析发现,7 种氨基酸、27 种有机酸、16 种糖和 22 种其他化合物的含量有显著差异,而转录组学数据显示,低温贮藏果实中有 1735 个差异表达基因(DEGs)下调,1369 个上调。我们发现,柠檬酸、顺乌头酸和琥珀酸的含量增加,这与冷处理番茄果实中 ATP-柠檬酸合酶(ACS)和异柠檬酸脱氢酶(IDH)基因的表达一致。冷胁迫促进 ACS 和 IDH 的表达,可能增加柠檬酸、顺乌头酸和琥珀酸的合成。丙氨酸和亮氨酸的含量增加,可能分别是由于丙氨酸氨基转移酶(ALT)和支链氨基酸氨基转移酶(BcAT)的高表达水平。总的来说,本研究中的转录组学和代谢组学数据解释了冷害的分子机制,并扩展了我们对番茄果实应对冷害时代谢网络复杂调控机制的理解。