Wang Lizhi, Liu Kaizhen, Wang Zhisheng, Bai Xue, Peng Quanhui, Jin Lei
Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, China.
Front Microbiol. 2019 Feb 20;10:239. doi: 10.3389/fmicb.2019.00239. eCollection 2019.
The objective of this study was to examine the association between bacterial community structure and the utilization efficiency of nitrogen (UEN) phenotypes by determining the bacterial community in the gastrointestinal tract (GIT) of goats that differ in UEN using high-throughput 16S rRNA gene sequencing. Thirty Nubian goats were selected as experimental animals, and their UEN was determined in a metabolic experiment. Subsequently, eight individuals were grouped into the high nitrogen utilization (HNU) phenotype, and seven were grouped into the low nitrogen utilization (LNU) phenotype. The bacterial 16S rRNA gene amplicons from the rumen, abomasum, jejunum, cecum and colon contents of these animals were sequenced using next-generation high-throughput sequencing technology. Two hundred thirty-nine genera belonging to 23 phyla in the rumen, 319 genera belonging to 30 phyla in the abomasum, 248 genera belonging to 36 phyla in the jejunum, 248 genera belonging to 25 phyla in the colon and 246 genera belonging to 23 phyla in the cecum were detected, with Bacteroidetes and Firmicutes predominating. In addition, a significant correlation was observed between the UEN and the genera and in the rumen; and in the abomasum; and in the jejunum; and in the colon; and in the cecum. Furthermore, the real-time PCR results showed that the ruminal copies of , , _sp._HUN007, and in the HNU animals were significantly higher than those in the LNU animals. This study suggests an association of GIT microbial communities as a factor that influences UEN in goats.
本研究的目的是通过使用高通量16S rRNA基因测序确定氮利用效率（UEN）不同的山羊胃肠道（GIT）中的细菌群落，来研究细菌群落结构与氮利用效率（UEN）表型之间的关联。选择30只努比亚山羊作为实验动物，并在代谢实验中测定它们的UEN。随后，8只个体被分为高氮利用（HNU）表型组，7只被分为低氮利用（LNU）表型组。使用下一代高通量测序技术对这些动物瘤胃、皱胃、空肠、盲肠和结肠内容物中的细菌16S rRNA基因扩增子进行测序。在瘤胃中检测到属于23个门的239个属，在皱胃中检测到属于30个门的319个属，在空肠中检测到属于36个门的248个属，在结肠中检测到属于25个门的248个属，在盲肠中检测到属于23个门的246个属，其中拟杆菌门和厚壁菌门占主导。此外，观察到瘤胃中的UEN与 属和 属之间存在显著相关性；皱胃中的UEN与 属和 属之间存在显著相关性；空肠中的UEN与 属和 属之间存在显著相关性；结肠中的UEN与 属和 属之间存在显著相关性；盲肠中的UEN与 属和 属之间存在显著相关性。此外，实时PCR结果表明，HNU动物瘤胃中 属、 属、_sp._HUN007属、 属和 属的拷贝数显著高于LNU动物。本研究表明，GIT微生物群落作为一个因素与山羊的UEN有关。
