基于rac融合启动子的表达载体。

Expression vectors based on the rac fusion promoter.

作者信息

Boros I, Lukacsovich T, Baliko G, Venetianer P

出版信息

Gene. 1986;42(1):97-100. doi: 10.1016/0378-1119(86)90154-x.

Abstract

The -35 region of the rrnB P2 promoter and the -10 region of the lacZpo promoter-operator were fused to form the strong and regulatable rac promoter. Vectors were constructed that allow the attachment of protein-coding sequences to the beta-galactosidase alpha-peptide (LacZ alpha) in any reading frame. By introducing a high-copy-number mutation, the synthesis of a LacZ alpha-chloramphenicol acetyltransferase fusion protein reached more than 60% of total cell protein in Escherichia coli.

摘要

rrnB P2启动子的 -35区域与lacZpo启动子-操纵子的 -10区域融合，形成了强且可调控的rac启动子。构建了一些载体，这些载体能使蛋白质编码序列以任何阅读框连接到β-半乳糖苷酶α-肽（LacZα）上。通过引入高拷贝数突变，LacZα-氯霉素乙酰转移酶融合蛋白的合成在大肠杆菌中达到了总细胞蛋白的60%以上。