Dialysis Room, Huaihe Hospital of Henan University, Kaifeng, Henan, China.
Department of Nephrology, Huaihe Hospital of Henan University, Kaifeng, Henan, China.
J Cell Biochem. 2019 Aug;120(8):13354-13360. doi: 10.1002/jcb.28609. Epub 2019 Mar 19.
Cryptotanshinone (CTS), an active component extracted from the root of Salvia miltiorrhiza Bunge , was reported to attenuate hepatic ischemia/reperfusion (I/R) injury. However, its protective effect against renal I/R injury remains unclear. In this study, the role of CTS in renal I/R injury in vitro and its possible mechanism were investigated. Our results showed that CTS improved cell viability in HK-2 cells exposed to hypoxia/reoxygenation (H/R). CTS also inhibited the H/R-mediated production of reactive oxygen species, as well as increased the activities of superoxide dismutase and catalase in H/R-stimulated HK-2 cells. In addition, CTS dramatically attenuated the induction of bax expression and caspase-3 activity and alleviated the reduction of bcl-2 expression in HK-2 cells cultured with H/R. Furthermore, CTS activated the levels of p-PI3K and p-Akt in H/R-injured HK-2 cells; meanwhile, the renal protective activity of CTS was inhibited by the inhibitor of the (phosphatidylinositol 3 kinase/protein kinase B) PI3K/Akt pathway (LY294002). These findings indicate that CTS can ameliorate renal I/R injury in vitro partly through regulating the PI3K/Akt pathway.
隐丹参酮（CTS）是从丹参根中提取的一种有效成分，据报道可减轻肝缺血/再灌注（I/R）损伤。然而，其对肾 I/R 损伤的保护作用尚不清楚。在本研究中，研究了 CTS 对体外肾 I/R 损伤的作用及其可能的机制。结果表明，CTS 可提高缺氧/复氧（H/R）暴露的 HK-2 细胞的细胞活力。CTS 还抑制了 H/R 介导的活性氧的产生，并增加了 H/R 刺激的 HK-2 细胞中超氧化物歧化酶和过氧化氢酶的活性。此外，CTS 可显著抑制 bax 表达和 caspase-3 活性的诱导，并减轻 H/R 培养的 HK-2 细胞中 bcl-2 表达的降低。此外，CTS 激活了 H/R 损伤的 HK-2 细胞中 p-PI3K 和 p-Akt 的水平；同时，PI3K/Akt 通路（LY294002）抑制剂抑制了 CTS 的肾保护活性。这些发现表明，CTS 可部分通过调节 PI3K/Akt 通路改善体外肾 I/R 损伤。
