Andersson D I, Andersson S G, Kurland C G
Biochimie. 1986 May;68(5):705-13. doi: 10.1016/s0300-9084(86)80164-x.
Here we show that ram mutations, either in ribosomal protein S4 or S5, decrease the proofreading flows for both cognate and noncognate ternary complexes bound by streptomycin-dependent (SmD) ribosomes. This effect is accompanied by a slight increase in the overall error frequency. More important, however, is the decreased proofreading of the cognate species which is almost reduced to wild-type levels. The data suggest that it may be the reduction of the proofreading of the cognate substrate that is important for suppressing streptomycin dependence. Furthermore, we show that rpsE mutants, selected from streptomycin-dependent strains, behave kinetically very similarly to the previously described rpsD mutants.
我们在此表明，核糖体蛋白S4或S5中的ram突变会降低由链霉素依赖型（SmD）核糖体结合的同源和非同源三元复合物的校对通量。这种效应伴随着总体错误频率的轻微增加。然而，更重要的是同源物种校对能力的下降，几乎降至野生型水平。数据表明，同源底物校对能力的降低可能对抑制链霉素依赖性很重要。此外，我们表明，从链霉素依赖型菌株中筛选出的rpsE突变体在动力学上与先前描述的rpsD突变体非常相似。
