Ahiskali Ibrahim, Pinar Can Lokman, Kiki Murat, Mammadov Renad, Ozbek Bilgin Asli, Hacimuftuoglu Ahmet, Cankaya Murat, Keskin Cimen Ferda, Altuner Durdu
a Department of Ophthalmology, Palandoken State Hospital , Erzurum , Turkey.
b Department of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University , Erzincan , Turkey.
Cutan Ocul Toxicol. 2019 Sep;38(3):227-232. doi: 10.1080/15569527.2019.1588289. Epub 2019 Mar 22.
Diabetic retinopathy (DR) is one of the leading causes of blindness. In DR patients, antioxidant defence is disrupted, and production of reactive oxygen species and pro-inflammatory cytokines such as interleukin 1β (IL-1β) and tumour necrosis factor alpha (TNF-α) increases. Taxifolin has been reported to suppress reactive oxygen species, IL-1β and TNF-α production. The aim of this study is to biochemically and histopathologically examine the protective effect of taxifolin against DR damage induced by alloxan. Alloxan received rats with a blood glucose level of ≥250 mg/dL were divided into taxifolin-treated (TAX) ( = 6), diabetic control (DC) ( = 6) groups. There were rats received only saline in non-diabetic control (NC) group ( = 6). Taxifolin (50 mg/kg) was orally administered to the TAX group rats. DC and NC rats received the same volume of saline as a solvent. This procedure was repeated once a day for 3 months. At the end of this period, animals were killed by high dose thiopental sodium anaesthesia. Histopathological examinations were then performed on excised rat eyes. Malondialdehyde (MDA), total glutathione (tGSH), IL-1β and TNF-α levels were measured in obtained blood samples. MDA, IL-1β and TNF-α levels were significantly increased in blood samples of DC group rats with hyperglycemia induced by alloxan compared with NC group ( < 0.0001), and decreased in the TAX group compared with the DC group ( < 0.0001). The levels of tGSH were significantly decreased in blood samples of DC group rats compared with NC group ( < 0.0001), and increased in the TAX group compared with the DC group ( < 0.0001). Histopathologically, retinal ganglion cells of the TAX group had a slightly dilated and congested blood vessel, and severe damage was inflicted to the retinal ganglion cell layer of the DC group. Experimental results suggest that taxifolin may be beneficial in the treatment of DR.
糖尿病视网膜病变（DR）是导致失明的主要原因之一。在DR患者中，抗氧化防御功能受到破坏，活性氧以及促炎细胞因子如白细胞介素1β（IL-1β）和肿瘤坏死因子α（TNF-α）的产生增加。据报道，花旗松素可抑制活性氧、IL-1β和TNF-α的产生。本研究旨在通过生物化学和组织病理学方法检测花旗松素对四氧嘧啶诱导的DR损伤的保护作用。将血糖水平≥250mg/dL的四氧嘧啶诱导糖尿病大鼠分为花旗松素治疗组（TAX）（n = 6）和糖尿病对照组（DC）（n = 6）。非糖尿病对照组（NC）（n = 6）的大鼠仅接受生理盐水。TAX组大鼠口服花旗松素（50mg/kg）。DC组和NC组大鼠接受相同体积的生理盐水作为溶剂。此操作每天重复一次，并持续3个月。在此期间结束时，通过高剂量硫喷妥钠麻醉处死动物。然后对切除的大鼠眼睛进行组织病理学检查。在采集的血液样本中测量丙二醛（MDA）、总谷胱甘肽（tGSH）、IL-1β和TNF-α水平。与NC组相比，DC组大鼠因四氧嘧啶诱导的高血糖导致血液样本中的MDA、IL-1β和TNF-α水平显著升高（P < 0.0001），与DC组相比，TAX组中这些指标降低（P < 0.0001）。与NC组相比，DC组大鼠血液样本中的tGSH水平显著降低（P < 0.0001），与DC组相比，TAX组中tGSH水平升高（P < 0.0001）。组织病理学检查显示，TAX组的视网膜神经节细胞血管轻度扩张和充血，而DC组的视网膜神经节细胞层受到严重损伤。实验结果表明，花旗松素可能对DR的治疗有益。
