a Global Centre for Environmental Remediation (GCER), Faculty of Science , The University of Newcastle , Callaghan , New South Wales , Australia.
b Cooperative Research Centre for Contamination Assessment and Remediation of Environment (CRC CARE) , The University of Newcastle , Callaghan , New South Wales , Australia.
Crit Rev Biotechnol. 2019 Aug;39(5):709-731. doi: 10.1080/07388551.2019.1597828. Epub 2019 Apr 11.
The excessive generation and discharge of wastewaters have been serious concerns worldwide in the recent past. From an environmental friendly perspective, bacteria, cyanobacteria and microalgae, and the consortia have been largely considered for biological treatment of wastewaters. For efficient use of bacteria‒cyanobacteria/microalgae consortia in wastewater treatment, detailed knowledge on their structure, behavior and interaction is essential. In this direction, specific analytical tools and techniques play a significant role in studying these consortia. This review presents a critical perspective on physical, biochemical and molecular techniques such as microscopy, flow cytometry with cell sorting, nanoSIMS and omics approaches used for systematic investigations of the structure and function, particularly nutrient removal potential of bacteria‒cyanobacteria/microalgae consortia. In particular, the use of specific molecular techniques of genomics, transcriptomics, proteomics metabolomics and genetic engineering to develop more stable consortia of bacteria and cyanobacteria/microalgae with their improved biotechnological capabilities in wastewater treatment has been highlighted.
在过去的一段时间里，过量的废水产生和排放已经引起了全世界的严重关注。从环保的角度来看，细菌、蓝藻和微藻以及它们的共生体已被广泛用于废水的生物处理。为了在废水处理中有效利用细菌-蓝藻/微藻共生体，详细了解它们的结构、行为和相互作用是必不可少的。在这方面，特定的分析工具和技术在研究这些共生体方面发挥了重要作用。本综述批判性地介绍了物理、生化和分子技术，如显微镜、带有细胞分选的流式细胞术、nanoSIMS 和组学方法，用于系统研究结构和功能，特别是细菌-蓝藻/微藻共生体的营养去除潜力。特别是，强调了使用基因组学、转录组学、蛋白质组学、代谢组学和遗传工程等特定分子技术来开发更稳定的细菌和蓝藻/微藻共生体，并提高其在废水处理中的生物技术能力。
