犬肥大细胞瘤中c-kit和TP53突变的患病率及预后价值

Prevalence and prognostic value of c-kit and TP53 mutations in canine mast cell tumours.

作者信息

Vozdova Miluse, Kubickova Svatava, Fictum Petr, Fröhlich Jan, Jelinek Frantisek, Rubes Jiri

机构信息

Central European Institute of Technology-Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.

出版信息

Vet J. 2019 May;247:71-74. doi: 10.1016/j.tvjl.2019.03.005. Epub 2019 Mar 21.

DOI:10.1016/j.tvjl.2019.03.005

PMID:30971355

Abstract

Cutaneous mast cell tumours (MCT) are among the most frequent malignancies in dogs. Their clinical behaviour is highly variable and, with the exception of mutations in the c-kit gene, little is known about their genetic aetiology. The mutational status of the c-kit exons 8, 9 and 11, and exons 5-8 of the TP53 gene was analysed to find markers for molecular stratification of MCTs and predictors of clinical outcome. Mutations in the c-kit gene were detected in 19.5% (n = 8/41) samples and their presence was significantly associated with the high histopathological grade (P = 0.038). Mutations in the DNA binding domain of the TP53 gene were found in 14.6% (n = 6/41) of the analysed MCTs, and their frequency was similar in low and high grade MCTs (P > 0.05). TP53 mutations were not useful prognostic factors in this sample of canine cutaneous MCTs.

摘要

皮肤肥大细胞瘤（MCT）是犬类最常见的恶性肿瘤之一。其临床行为高度可变，除了c-kit基因的突变外，对其遗传病因知之甚少。分析了c-kit基因外显子8、9和11以及TP53基因外显子5-8的突变状态，以寻找MCT分子分层的标志物和临床结果的预测指标。在19.5%（n = 8/41）的样本中检测到c-kit基因突变，其存在与高组织病理学分级显著相关（P = 0.038）。在14.6%（n = 6/41）的分析MCT中发现了TP53基因DNA结合域的突变，其在低级别和高级别MCT中的频率相似（P > 0.05）。在该犬皮肤MCT样本中，TP53突变不是有用的预后因素。