文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

SRC-1 和 Twist1 高表达预示着不良预后，并通过诱导人鼻咽癌中的上皮-间充质转化促进迁移和侵袭。

High SRC-1 and Twist1 expression predicts poor prognosis and promotes migration and invasion by inducing epithelial-mesenchymal transition in human nasopharyngeal carcinoma.

机构信息

Department of Otorhinolaryngology, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, China.

Department of Otorhinolaryngology, Peking University Shenzhen Hospital, Shenzhen, China.

出版信息

PLoS One. 2019 Apr 11;14(4):e0215299. doi: 10.1371/journal.pone.0215299. eCollection 2019.

DOI:10.1371/journal.pone.0215299
PMID:30973923
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6459498/
Abstract

Steroid receptor coactivator 1 (Src-1) and Twist1 are aberrantly upregulated in a variety of tumors and play an important role in tumor progression. However, the exact role of Src-1 and Twist1 in nasopharyngeal carcinoma (NPC) is uncertain. In this study, we investigated the possible prognostic value and biological effect of Src-1 and Twist1 in NPC. Src-1 and Twist1 expression was detected in a cohort of NPC patients (n = 134) by qRT-PCR. Kaplan-Meier survival analysis was used comparing overall survival (OS) and progression-free survival (PFS). Multivariate analysis was performed using the Cox proportional hazard regression model. Biologic effect of Src-1 and Twist1 in NPC cell lines was evaluated by western blot, colony formation assay, soft agar assay, scratch wound healing assay, transwell invasion assay and tumor xenografts growth. We have found that Src-1 and Twist1 were aberrantly upregulated in human NPC tissues, and associated with advanced tumor stage, distant metastasis and unfavorable prognosis. Knockdown of Src-1 or Twist1 in human NPC cell line CNE-1 suppressed colony formation, anchorage-independent growth, cell migration, invasion and tumor xenografts growth, while enforced expression of Src-1 or Twist1 in human NPC cell line HNE-2 promotes anchorage-independent growth, cell migration and invasion. In addition, Src-1 and Twist1 could suppress E-cadherin expression and increase Vimentin expression, thus suggested that Src-1 and Twist1 enhanced the malignant behaviors of NPC cells via inducing epithelial-mesenchymal transition (EMT). Our data indicated that Src-1 and Twist1 could be possible prognostic biomarkers and potential therapy targets for patients with NPC.

摘要

类固醇受体共激活因子 1（Src-1）和 Twist1 在多种肿瘤中异常上调，在肿瘤进展中发挥重要作用。然而，Src-1 和 Twist1 在鼻咽癌（NPC）中的确切作用尚不确定。在这项研究中，我们研究了 Src-1 和 Twist1 在 NPC 中的可能预后价值和生物学效应。通过 qRT-PCR 检测了一组 NPC 患者（n=134）中 Src-1 和 Twist1 的表达。使用 Kaplan-Meier 生存分析比较总生存期（OS）和无进展生存期（PFS）。使用 Cox 比例风险回归模型进行多变量分析。通过 Western blot、集落形成实验、软琼脂实验、划痕愈合实验、Transwell 侵袭实验和肿瘤异种移植生长实验评估 Src-1 和 Twist1 在 NPC 细胞系中的生物学效应。我们发现 Src-1 和 Twist1 在人 NPC 组织中异常上调，并与晚期肿瘤分期、远处转移和不良预后相关。在人 NPC 细胞系 CNE-1 中敲低 Src-1 或 Twist1 抑制集落形成、锚定非依赖性生长、细胞迁移、侵袭和肿瘤异种移植生长，而在人 NPC 细胞系 HNE-2 中强制表达 Src-1 或 Twist1 促进锚定非依赖性生长、细胞迁移和侵袭。此外，Src-1 和 Twist1 可以抑制 E-钙粘蛋白的表达并增加波形蛋白的表达，从而提示 Src-1 和 Twist1 通过诱导上皮-间充质转化（EMT）增强 NPC 细胞的恶性行为。我们的数据表明，Src-1 和 Twist1 可能是 NPC 患者的可能预后生物标志物和潜在治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/983c/6459498/d43be7664823/pone.0215299.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/983c/6459498/a1d97f861b4d/pone.0215299.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/983c/6459498/a9657e46b044/pone.0215299.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/983c/6459498/9c9381c64a8f/pone.0215299.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/983c/6459498/4d5b54e7b1d5/pone.0215299.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/983c/6459498/59a5e3775e4f/pone.0215299.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/983c/6459498/d43be7664823/pone.0215299.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/983c/6459498/a1d97f861b4d/pone.0215299.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/983c/6459498/a9657e46b044/pone.0215299.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/983c/6459498/9c9381c64a8f/pone.0215299.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/983c/6459498/4d5b54e7b1d5/pone.0215299.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/983c/6459498/59a5e3775e4f/pone.0215299.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/983c/6459498/d43be7664823/pone.0215299.g006.jpg

相似文献

[1]
High SRC-1 and Twist1 expression predicts poor prognosis and promotes migration and invasion by inducing epithelial-mesenchymal transition in human nasopharyngeal carcinoma.

PLoS One. 2019-4-11

[2]
c-Src activation promotes nasopharyngeal carcinoma metastasis by inducing the epithelial-mesenchymal transition via PI3K/Akt signaling pathway: a new and promising target for NPC.

Oncotarget. 2016-5-10

[3]
Knockdown of long non-coding RNA TUG1 suppresses nasopharyngeal carcinoma progression by inhibiting epithelial-mesenchymal transition (EMT) via the promotion of miR-384.

Biochem Biophys Res Commun. 2018-12-21

[4]
Downregulating HMGA2 attenuates epithelial-mesenchymal transition-induced invasion and migration in nasopharyngeal cancer cells.

Biochem Biophys Res Commun. 2015-7-31

[5]
Increased expression of lncRNA SNHG12 predicts a poor prognosis of nasopharyngeal carcinoma and regulates cell proliferation and metastasis by modulating Notch signal pathway.

Cancer Biomark. 2018

[6]
Targeting FoxM1 inhibits proliferation, invasion and migration of nasopharyngeal carcinoma through the epithelial‑to-mesenchymal transition pathway.

Oncol Rep. 2015-5

[7]
Overexpression of Nogo receptor 3 (NgR3) correlates with poor prognosis and contributes to the migration of epithelial cells of nasopharyngeal carcinoma patients.

J Mol Med (Berl). 2018-1-11

[8]
Human/eukaryotic ribosomal protein L14 (RPL14/eL14) overexpression represses proliferation, migration, invasion and EMT process in nasopharyngeal carcinoma.

Bioengineered. 2021-12

[9]
FOXA1 Expression in Nasopharyngeal Carcinoma: Association with Clinicopathological Characteristics and EMT Markers.

Biomed Res Int. 2020

[10]
MicroRNA-296-5p inhibits cell metastasis and invasion in nasopharyngeal carcinoma by reversing transforming growth factor-β-induced epithelial-mesenchymal transition.

Cell Mol Biol Lett. 2020-11-3

引用本文的文献

[1]
Mesenchymal Stem Cell-conditioned Medium Protecting Renal Tubular Epithelial Cells by Inhibiting Hypoxia-inducible Factor-1α and Nuclear Receptor Coactivator-1.

Curr Stem Cell Res Ther. 2024

[2]
Src-1 and SP2 promote the proliferation and epithelial-mesenchymal transition of nasopharyngeal carcinoma.

Open Med (Wars). 2021-7-15

[3]
NF-κB/TWIST1 Mediates Migration and Phagocytosis of Macrophages in the Mice Model of Implant-Associated Osteomyelitis.

Front Microbiol. 2020-6-12

[4]
Contrast-enhanced Ultrasound in evaluating of angiogenesis and tumor staging of nasopharyngeal carcinoma in nude mice.

PLoS One. 2019-8-23

本文引用的文献

[1]
Twist1 Regulates Vimentin through Cul2 Circular RNA to Promote EMT in Hepatocellular Carcinoma.

Cancer Res. 2018-5-29

[2]
Nasopharyngeal carcinoma.

Lancet. 2015-8-28

[3]
The soft agar colony formation assay.

J Vis Exp. 2014-10-27

[4]
SRC-1 and Twist1 expression positively correlates with a poor prognosis in human breast cancer.

Int J Biol Sci. 2014-3-15

[5]
Global gene repression by the steroid receptor coactivator SRC-1 promotes oncogenesis.

Cancer Res. 2014-3-19

[6]
The battle against nasopharyngeal cancer.

Radiother Oncol. 2012-8-30

[7]
Normal and disease-related biological functions of Twist1 and underlying molecular mechanisms.

Cell Res. 2011-8-30

[8]
HOXC11-SRC-1 regulation of S100beta in cutaneous melanoma: new targets for the kinase inhibitor dasatinib.

Br J Cancer. 2011-6-7

[9]
How does intensity-modulated radiotherapy versus conventional two-dimensional radiotherapy influence the treatment results in nasopharyngeal carcinoma patients?

Int J Radiat Oncol Biol Phys. 2010-7-17

[10]
A genome-wide association study of nasopharyngeal carcinoma identifies three new susceptibility loci.

Nat Genet. 2010-5-30

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索